DEVELOPMENT OF NEURAL CELL LINES FOR TRANSPLANTATION

这项研究的主要目标是获得明确的、 用于脑植入的替代神经组织来源 治疗帕金森氏症等中枢神经系统疾病 疾病。将检查神经细胞的三个来源和 在体外进行调节，以建立这些细胞的潜能 宿主大脑内的整合和稳定性。(I)设立 神经细胞系将在体外用抗有丝分裂分化 药物、维生素A类似物和生长调节剂，并研究 细胞表面特性和细胞周期动力学。(Ii)多巴胺能 有丝分裂的细胞融合将产生神经细胞杂交体 神经胶质细胞与胚胎黑质细胞建立长 中枢多巴胺神经元的连续培养。这些 还将对细胞表面属性进行筛选，这些属性 脑内潜在整合的重要标准 薄壁组织。(Iii)此外，人和猴子的肾上腺髓质 将从器官捐赠中获得并在体外培养 生长因子，并与产生生长的细胞共培养 各种因素。长期培养将以神经细胞为特征 性质、移植抗原的表达和 神经肽在这个潜在的可移植组织中的共存 组织。 这些研究的结果将使定制的神经细胞成为可能 用于移植到具有最佳特征的灵长类动物和 将为修复人类中枢神经系统奠定基础 神经退行性疾病，如帕金森氏症。

The primary goal of this research is to obtain well defined, alternate sources of neural tissue for brain implantation to remedy central nervous system disorders such as Parkinson's disease. Three sources of neuronal cells will be examined and modulated in vitro to establish the potential of these cells for integration and stability within the host brain. (i) Established neuronal cell lines will be differentiated in vitro with antimitotic agents, vitamin A analogs and growth modulators and studied for cell surface properties and cell cycle kinetics. (ii) Dopaminergic neuronal cell hybrids will be produced by cell fusion of mitotic glial cells with embryonic substantia nigra cells to establish long- term, continuous, cultures of central dopamine neurons. These cells will also be screened for cell surface properties which are important criteria for potential integration within brain parenchyma. (iii) In addition, human and monkey adrenal medulla will be obtained from organ donation and cultured in vitro with growth factors and cocultured with cells which produce growth factors. Long-term cultures will be characterized for neuronal properties, the expression of transplantation antigens and colocalization of neuropeptides in this potentially transplantable tissue. Results of these studies will make available neural cells tailored for transplantation into primates with optimal characteristics and will lay the groundwork for repair of human CNS neurodegenerative diseases such as Parkinson's disease.