ANTI-HIV POTENTIAL OF PENTOSAN POLYSULFATE

戊聚糖多硫酸酯的抗 HIV 潜力

• 批准号: 3901023
• 负责人: L B BIGELOW
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3901023
• 关键词: AIDS; AIDS therapy; antiAIDS agent; antiviral agents; combination chemotherapy; dextrans; human immunodeficiency virus; human subject; human therapy evaluation; pentosan; polyanion; sulfates; virus replication; zidovudine

Pentosan polysulfate (PPS), a semi-synthetic polyanion, has been under investigation here for a number of years as a prophylactic treatment for migraine and cluster headache. Since we observed an increase of circulating T-cells in treated patients and because of the reported synergism of another sulfated polyanion, dextran sulfate, with AZT, we have performed in-vitro experiments to evaluate the therapeutic potential of this drug. Using human peripheral blood mononuclear cells we have been able to demonstrate synergism of PPS with aziduvine (AZT) in the suppression of HIV-1 growth. We also observed that PPS and dextran sulfate alone in concentrations less than 1.Omug/ml stimulated viral replication. This latter finding is particularly important because of the reported "underground market" for dextran sulfate. It suggests that persons with AIDS could actually worsen their condition if they were to take polyanions such as dextran. On the other hand, the finding of a powerful synergism between AZT and PPS gives encouragement to careful design of clinical research to evaluate this substance as an adjunct to the treatment of AIDS that might permit effective lower dosing of AZT.

戊聚糖多硫酸酯（PPS）是一种半合成聚阴离子，已被研究 作为预防性治疗，这里进行了多年的调查 偏头痛和丛集性头痛。 由于我们观察到增加 治疗患者体内的循环 T 细胞，并且由于报道 另一种硫酸化聚阴离子硫酸葡聚糖与 AZT 的协同作用，我们有 进行体外实验以评估其治疗潜力 这种药。 利用人外周血单核细胞，我们能够 证明 PPS 与叠氮文 (AZT) 在抑制 HIV-1 生长。 我们还观察到单独使用 PPS 和硫酸葡聚糖 浓度低于1.0mug/ml刺激病毒复制。 后一个发现尤其重要，因为据报道 硫酸葡聚糖的“地下市场”。 它表明，患有以下疾病的人 如果他们服用聚阴离子，艾滋病实际上可能会使他们的病情恶化 如右旋糖酐。 另一方面，发现 AZT 和 PPS 之间存在强大的协同作用 鼓励仔细设计临床研究来评估这一点 物质作为艾滋病治疗的辅助手段，可能允许 有效降低 AZT 剂量。