NEUROCHEMICAL ANALYZER

麻省理工学院神经科学系成员 医学目前正在进行小灵通资助的临床和基础 关于糖尿病发病机制和治疗的研究计划 包括阿尔茨海默氏症在内的退行性中枢神经系统疾病 疾病、帕金森症、癫痫以及对正常衰老的研究。 这些研究的一个主要方面涉及神经递质， 使用高性能的神经肽、氨基酸和药物分析 高效液相色谱-电化学检测(LC-EC) 仪器仪表。大多数化验都是通过 与附近的调查人员合作审问 机构。目前和未来的人类和动物模型研究， 然而，这涉及更多的样本和测量的需要 其他重要的神经化学物质或药物物质，从而使 这些协作安排不切实际，也不充分。这个 需要一种具有更高分析分辨率的仪器 为了准确地分析复杂的生物组织(例如，人脑， 脑脊液和血浆)，同时能够处理大量 样本数。神经化学分析仪(NCA)， ESA，Inc.新近开发的高效液相色谱-电化学仪 符合这些标准。购买NCA需要资金 作为部门的“核心”设备，共同使用 它资助的调查人员。与其他高效液相色谱-EC系统不同的是，NCA 结合：i)卓越的分析分辨率通过其独特的 16个电化学检测器阵列；ii)能力 在一次45分钟内量化超过80种化合物；iii)高 通过其冷冻的100个样品自动进样器和 计算机驱动，24小时(通宵)无人值守操作；以及 常规敏感度在中等范围内(5×10-13)。A五 成员内部顾问委员会将监督其使用。在.期间 头两年，三大用户将100%利用这些资源 仪器的时间以一周五天24小时工作制为基础。在……里面 随后的几年，随着时间的推移，其他八个 部门调查人员将被允许使用该仪器 用于他们的研究项目中涉及的神经化学分析。 PI在开发分析软件方面拥有丰富的经验 方法使用高效液相色谱-电化学仪，并将培训和监督 用户技术人员对NCA的操作和使用。

Members of the Department of Neurosciences at the School of Medicine are currently conducting PHS funded clinical and basic research programs concerning the mechanisms and treatment of degenerative central nervous system diseases including Alzheimer's disease, Parkinsonism, epilipsy as well as studies of normal aging. A major aspect of these studies involves neurotransmitter, neuropeptide, amino acid, and drug analyses using high-performance liquid chromatography, electrochemical detection (HPLC-EC) instrumentation. The majority of assays have been provided through collaborative arragnements with investigators at nearby institutions. Current and future human and animal model studies, however, involve larger numbers of samples and the need to measure other important neurochemical or drug substances, thus rendering these collaborative arrangements impractical and inadequate. The need exists for an instrument with superior analytical resolution to accurately assay complex biological tissues (e.g., human brain, CSF, and plasma) and at the same time be able to process large numbers of samples. The Neurochem Neurochemical Analyzer (NCA), an HPLC-EC instrument recently developed by ESA, Inc. uniquely fulfills these criteria. Funds are requested to purchase the NCA to serve as a departmental "core" apparatus for the shared use of its funded investigators. Unlike other HPLC-EC systems, the NCA combines: i) a superior analytical resolution through its unique array of 16 electrochemical detectors; ii) capability of quantifying over 80 compounds in a single 45 mins run; iii) high volume capacity through its refrigerated 100 sample autosampler and computer driven, 24 hr (overnight) unattended operation; and iv) routine sensitivity in the mid femtogram (5 X 10-13) range. A five member internal advisory board will oversee its use. During the first two years the three major user will utilize 100% of this instrument's time based on a 24 hr, five day work week. In subsequent years, as time becomes available, eight other departmental investigators will be permitted to use this instrument for neurochemical analyses involved in their research projects. The PI has extensive experience in the development of analytical methods using HPLC-EC instruments and will train and supervise the users' technicians in the operation and use of the NCA.