CIRCULAR DICHROISM SPECTROPOLARIMETER

圆二向色性(CD)测量是最有价值的测量之一 关于解决方案的结构和构象信息的来源 化学和生化化合物。最近在以下方面的改进 工具进一步增强了这一功能的有用性。 化学和生物化学研究中的技术。这项建议是 购买一台现代化的CD光谱偏振仪，供 一大批研究人员。委员会正在调查的问题 主要用户群是1)肌球蛋白、肌动蛋白和肌肉的结构 收缩，2)泛素蛋白的结构分析 偶联物，3)药物分子和致癌物与 双链DNA，4)蜂毒素和多肽的齐聚 来自HIV gp41，5)眼晶状体蛋白和其他 视觉系统，6)α-螺旋的静电性质，7) 载脂蛋白B在腰椎手术中的二维结构 低密度脂蛋白，8)分子分析、合成和 防御素的折叠，以及9)序列特异性的合成 核酸酶。拟议购买CD设备将实现 NIH对这类保险的迫切研究需求- 支持加州大学洛杉矶分校的调查人员。

Circular dichroism (CD) measurements are one of the most valuable sources of structural and conformational information on solutions of chemical and biochemical compounds. Recent improvements in instrumentation have further enhanced the usefulness of this technique in chemical and biochemical research. This proposal is for the purchase of a modern CD spectropolarimeter to be used by a large number of researchers. Problems under investigation by the prime user group are 1) structures of myosin and actin and muscle contraction, 2) structural analysis of ubiquitin-protein conjugates, 3) complexes of drug molecules and carcinogens with double stranded DNA, 4) oligomerization of melittin and peptides from HIV gp41, 5) eye lens proteins and other proteins of the visual system, 6) electrostatic properties of the alpha-helix, 7) two dimensional structure of apolipoprotein B on the surgace of the low density lipoprotein, 8) molecular analysis, synthesis, and folding of defensins, and 9) synthesis of sequence specific nucleases. The proposed purchase of CD equipment will fulfill acute research needs for this type of insurumentation by NIH- supported investigators at UCLA.