CIRCULAR DICHROISM SPECTROPOLARIMETER

圆二色性（CD）测量是最有价值的测量之一。 溶液结构和构象信息的来源 化学和生物化学化合物。 最近有所改善 仪器进一步增强了这一有用性， 化学和生物化学研究技术。 这项建议是 购买一台现代CD分光偏振仪，供 大量的研究人员。 调查中的问题 主要用户群是1）肌球蛋白和肌动蛋白以及肌肉的结构 收缩，2）泛素蛋白的结构分析 缀合物，3）药物分子和致癌物的复合物， 双链DNA，4）蜂毒肽和肽的寡聚化 来自HIV gp 41，5）眼透镜蛋白和其他蛋白质 视觉系统，6）α-螺旋的静电特性，7） 载脂蛋白B的二维结构 低密度脂蛋白，8）分子分析，合成，和 防御素的折叠，和9）序列特异性的 核酸酶 购买CD设备的建议将满足 美国国立卫生研究院对这种保险的迫切研究需求- 支持加州大学洛杉矶分校的调查人员。

Circular dichroism (CD) measurements are one of the most valuable sources of structural and conformational information on solutions of chemical and biochemical compounds. Recent improvements in instrumentation have further enhanced the usefulness of this technique in chemical and biochemical research. This proposal is for the purchase of a modern CD spectropolarimeter to be used by a large number of researchers. Problems under investigation by the prime user group are 1) structures of myosin and actin and muscle contraction, 2) structural analysis of ubiquitin-protein conjugates, 3) complexes of drug molecules and carcinogens with double stranded DNA, 4) oligomerization of melittin and peptides from HIV gp41, 5) eye lens proteins and other proteins of the visual system, 6) electrostatic properties of the alpha-helix, 7) two dimensional structure of apolipoprotein B on the surgace of the low density lipoprotein, 8) molecular analysis, synthesis, and folding of defensins, and 9) synthesis of sequence specific nucleases. The proposed purchase of CD equipment will fulfill acute research needs for this type of insurumentation by NIH- supported investigators at UCLA.