CIRCULAR DICHROISM SPECTROPOLARIMETER

圆二色性 (CD) 测量是最有价值的测量之一 解决方案的结构和构象信息来源 化学和生化化合物。 最近的改进 仪器的使用进一步增强了该方法的实用性 化学和生物化学研究技术。 这个提议是 购买现代 CD 分光旋光计供以下人员使用 大量的研究人员。 调查组正在调查的问题 主要用户群是 1) 肌球蛋白、肌动蛋白和肌肉的结构 收缩，2）泛素蛋白的结构分析 缀合物，3）药物分子和致癌物的复合物 双链 DNA，4) 蜂毒肽和肽的寡聚化 来自 HIV gp41，5) 眼晶状体蛋白和其他蛋白质 视觉系统，6) α-螺旋的静电特性，7) 载脂蛋白B表面的二维结构 低密度脂蛋白，8) 分子分析、合成和 防御素的折叠，以及 9) 序列特异性的合成 核酸酶。 拟议购买 CD 设备将满足 NIH 对此类保险的迫切研究需求 支持加州大学洛杉矶分校的研究人员。

Circular dichroism (CD) measurements are one of the most valuable sources of structural and conformational information on solutions of chemical and biochemical compounds. Recent improvements in instrumentation have further enhanced the usefulness of this technique in chemical and biochemical research. This proposal is for the purchase of a modern CD spectropolarimeter to be used by a large number of researchers. Problems under investigation by the prime user group are 1) structures of myosin and actin and muscle contraction, 2) structural analysis of ubiquitin-protein conjugates, 3) complexes of drug molecules and carcinogens with double stranded DNA, 4) oligomerization of melittin and peptides from HIV gp41, 5) eye lens proteins and other proteins of the visual system, 6) electrostatic properties of the alpha-helix, 7) two dimensional structure of apolipoprotein B on the surgace of the low density lipoprotein, 8) molecular analysis, synthesis, and folding of defensins, and 9) synthesis of sequence specific nucleases. The proposed purchase of CD equipment will fulfill acute research needs for this type of insurumentation by NIH- supported investigators at UCLA.