RECEPTOR MEDIATED T CELL ACTIVATION

受体介导的 T 细胞激活

• 批准号: 3939369
• 负责人: R J HODES
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939369
• 关键词: T lymphocyte; antigen antibody reaction; antileukocyte isoantibody; clone cells; exocytosis; granule; leukocyte activation /transformation; monoclonal antibody

The mechanism of T cell activation has been studied employing both cloned T cell populations and naive heterogeneous T cell populations. Comparison has been made of T cell stimulation by antibodies directed to allotypic determinants on the T cell receptor, T cell activation by specific antigen, and T cell activation by non-receptor-mediated signaling. It has been demonstrated that both cloned and naive T cells can be triggered by the monoclonal antibody F23.1, directed toward determinants on the T cell receptor. Naive Lyt2+ T cells were activated to proliferate in the presence of soluble F23.1, IL-2, and accessory cells. Under the same conditions, L3T4+ naive T cells were unresponsive. These findings thus demonstrated a difference in the activation requirements of T cell subpopulations triggered through T cell receptor determinants. Specific "targeting" of the T cell receptor to accessory cell structures by heteroaggregates of F23.1 coupled to monoclonal anti-Ia antibody were, in contrast, capable of activating both Lyt2+ and L3T4+ T cell subpopulations. The nature of activation signals provided under these diverse conditions is currently under study. In particular, the function of endogenous lymphokines is being analyzed. Parameters of T cell response including specific gene activation and exocytosis of cytoplasmic granules in response to diverse stimuli have been evaluated.

T细胞活化的机制已被研究，采用 克隆的T细胞群和幼稚异质性T细胞群两者均 人口。 已经比较了T细胞刺激， 针对T细胞上同种异型决定簇的抗体 受体、特异性抗原对T细胞的活化及T细胞 通过非受体介导的信号传导激活。 已经 表明克隆和初始T细胞都可以被触发， 通过单克隆抗体F23.1，针对决定簇 在T细胞受体上。 初始Lyt 2 + T细胞被激活， 在可溶性F23.1、IL-2和辅助因子的存在下增殖 细胞 在相同的条件下，L3 T4+初始T细胞被激活。 反应迟钝 因此，这些发现表明， T细胞亚群的激活要求触发了 通过T细胞受体决定簇。 具体的“目标”， 通过异源聚集体的辅助细胞结构的T细胞受体 与之相反， 能够激活Lyt 2+和L3 T4 + T细胞亚群。 在这些不同的条件下提供的激活信号的性质 条件目前正在研究中。特别是， 正在分析内源性淋巴因子。 T细胞参数 包括特异性基因激活和胞吐的反应 细胞质颗粒对不同刺激的反应， 评估。