RECEPTOR MEDIATED T CELL ACTIVATION

受体介导的 T 细胞激活

• 批准号: 3963093
• 负责人: R J HODES
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963093
• 关键词: antigen antibody reaction; leukocyte activation /transformation; monoclonal antibody; neoplasm /cancer immunotherapy

The mechanism of T cell activation has been studied employing both cloned T cell populations and naive heterogeneous T cell populations. Comparison has been made of T cell stimulation by antibodies directed to allotypic determinants on the T cell receptor, T cell activation by specific antigen, and T cell activation by non-receptor-mediated signaling. It has been demonstrated that both cloned and naive T cells can be triggered by the monoclonal antibody F23.1, directed toward determinants on the T cell receptor. Naive Lyt2+ T cells were activated to proliferate in the presence of soluble F23.1, IL-2, and accessory cells. Under the same conditions, L3T4+ naive T cells were unresponsive. These findings thus demonstrated a difference in the activation requirements of T cell subpopulations triggered through T cell receptor determinants. Specific "targeting" of the T cell receptor to accessory cell structures by heteroaggregates of F23.1 coupled to monoclonal anti-Ia antibody were, in contrast, capable of activating both Lyt2+ and L3T4+ T cell subpopulations. The nature of activation signals provided under these diverse conditions is currently under study.

T细胞活化的机制已经使用克隆的T细胞和T细胞活化的T细胞进行了研究。 细胞群和幼稚异质T细胞群。 比较 由针对同种异型抗体的T细胞刺激制成， T细胞受体上的决定簇，特异性抗原对T细胞的活化， 和通过非受体介导的信号传导的T细胞活化。 已经 证明克隆和幼稚T细胞都可以被激活。 单克隆抗体F23.1，针对T细胞上的决定簇 受体的 初始Lyt 2 + T细胞被激活以在细胞中增殖。 存在可溶性F23.1、IL-2和辅助细胞。 根据同一 在这种条件下，L3 T4+初始T细胞是无应答的。 因此，这些发现 证明了T细胞活化要求的差异， 通过T细胞受体决定簇触发的亚群。 具体 T细胞受体“靶向”辅助细胞结构， 与单克隆抗Ia抗体偶联的F23.1的异聚集体， 相反，能够激活Lyt 2+和L3 T4 + T细胞 亚群 在这些条件下提供的激活信号的性质 目前正在研究各种条件。