CARCINOGENESIS OF MAMMALIAN CELLS IN CULTURE

皮肤成纤维细胞和外周血淋巴细胞的培养 正常和癌症易感个体，以及肿瘤细胞 在培养物中或体内转化，用于评估 辐射诱导的染色体DNA 损伤，DNA修复缺陷，癌症易感性和 恶性肿瘤转化 的发生率增加 X射线照射后的G-2期染色单体损伤 细胞周期与癌症易感性和 恶性转化，并可提供测试的基础， 癌症易感性 这种辐射敏感性的遗传基础 表明基因定位于特定染色体 从体细胞杂交的研究，小鼠的近交系， 同类小鼠品系。 染色体辐射敏感性 似乎是由G-2期DNA修复缺陷引起的。 另一 这个项目的一个方面是开发一个可复制的转换 人表皮角质形成细胞体外模型 用于跟踪生物和生化变化的进展 导致肿瘤转化。 相关的问题是 确定可定量细胞形态学变化， 肿瘤转化以促进转染， 转型研究。

Cultures of skin fibroblasts and peripheral lymphocytes from normal and cancer-prone individuals, as well as neoplastic cells transformed in culture or in vivo, are utilized in evaluating the relationship between radiation-induced chromosomal DNA damage, deficient DNA repair, cancer susceptibility and malignant neoplastic transformation. An increased incidence of chromatid damage after x-irradiation during the G-2 phase of the cell cycle is associated with both a predisposition to cancer and malignant transformation and can provide the basis of a test for cancer susceptibility. A genetic basis for this radiosensitivity with localization of genes to specific chromosomes is indicated from studies with somatic cell hybrids, inbred strains of mice, and congenic mouse strains. The chromosomal radiosensitivity appears to result from deficient DNA repair during G-2. Another aspect of this project is to develop a reproducible transformation system with human epidermal keratinocytes as an in vitro model for following the progression of biologic and biochemical changes leading to neoplastic transformation. An associated problem is to identify quantifiable cytomorphologic changes diagnostic of neoplastic transformation to facilitate transfection and transformation studies.