CARCINOGENESIS OF MAMMALIAN CELLS IN CULTURE

培养正常人和癌症高发者的皮肤成纤维细胞，如 以及在培养或体内转化的肿瘤细胞，用于 评价辐射诱发的染色体DNA与染色体DNA的关系 损伤、DNA修复缺陷、癌症易感性和恶性 肿瘤性转化。染色单体损伤的发生率增加 X-射线照射后细胞周期的GS期与 既有患癌症和恶变的倾向，也有可能 为癌症易感性测试提供依据。一种遗传基础 因为这种辐射敏感性来自对体细胞的研究 正常细胞和恶性细胞之间的杂交。染色体 放射敏感性似乎是由于G2期间DNA修复不足所致。 这个项目的另一个方面是开发一种可重现的转换 人表皮角质形成细胞体外模型的建立 随着生物和生化变化的进展导致 肿瘤性转化。一个相关的问题是识别 肿瘤转化的细胞形态改变诊断 促进转基因和转化研究。努力正在进行中 增强型G2染色体生化相关性研究进展 放射敏感性为癌症敏感性提供更快速的检测方法 并了解癌症易感人群的DNA修复缺陷。

Cultures of skin fibroblasts from normal and cancer-prone individuals, as well as neoplastic cells transformed in culture or in vivo, are utilized in evaluating the relationship between radiation-induced chromosomal DNA damage, deficient DNA repair, cancer susceptibility and malignant neoplastic transformation. An increased incidence of chromatid damage after x-irradiation during GS phase of the cell cycle is associated with both a predisposition to cancer and malignant transformation and may provide the basis for a test for cancer susceptibility. A genetic basis for this rediosensitivity is indicated from studies with somatic cell hybrids between normal and malignant cells. The chromosomal radiosensitivity appears to result from deficient DNA repair during G2. Another aspect of this project is to develop a reproducible transformation system with human epidermal keratinocytes as an in vitro model for following the progression of biologic and biochemical changes leading to neoplastic transformation. An associated problem is to identify cytomorphologic changes diagnostic of neoplastic transformation to facilitate transfection and transformation studies. Efforts are in progress to develop a biochemical correlate of enhanced G2 chromosomal radiosensitivity to provide a more rapid assay for cancer susceptibility and to understand the DNA repair deficiencies of cancer-prone individuals.