POLYPEPTIDE MODULATION IN MCF-7 CELLS BY ESTROGEN AND GROWTH FACTORS

雌激素和生长因子对 MCF-7 细胞中多肽的调节

• 批准号: 3939733
• 负责人: S S THORGEIRSSON
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939733
• 关键词: breast neoplasms; estrogen inhibitor; estrogens; gene expression; growth factor; hormone regulation /control mechanism; insulinlike factor; neoplastic cell; oncogenes; tissue /cell culture; transforming growth factors

The purpose of this project was to utilize the established human mammary tumor cell lines (MCF-7, MCF-7(gpt) (produced by transfection with the Eco-gpt selectable gene marker), MCF- 7(ras) (produced by transfection with Eco-gpt and the v-Hras oncogene) and LY2) to investigate the effect of antiestrogens, estrogen and other growth factors on the polypeptide expression of these cells. The growth factors IGF-1 (insulin-like growth factor-1) and TGF-alpha (transforming growth factor alpha) are able to elicit many of the growth stimulating responses of estrogen when applied to the MCF-7 human mammary tumor cell line. An initial baseline study between the MCF-7, MCF-7(gpt) and MCF-7(ras) has found that there are several polypeptides expressed only in MCF-7 (14), MCF-7(gpt) (5) and MCF-7(ras) (3). A number of quantitative differences between the cell lines were apparent, with the major differences occurring between the MCF-7 - MCF-7(gpt) and MCF-7 - MCF-7(ras). Studies to assess the effect of estrogen on the cellular polypeptide expression and the effect of estrogen, IGF-1 and TGF-alpha on cellular and secreted proteins of the MCF-7, MCF-7(gpt) and MCF-7(ras) are in the final analysis stage. Additional to this are experiments using the antiestrogen LY117018 to assess the effect on polypeptide expression and secretion from the antiestrogen- resistant cell line LY2 and the MCF-7 cells.

该项目的目的是利用现有的人类 乳腺肿瘤细胞系（MCF-7，MCF-7（gpt）（由 用Eco-gpt选择性基因标记物转染），MCF- 7（ras）（通过用Eco-gpt和v-Hras转染产生 癌基因）和LY 2）来研究抗雌激素的作用， 雌激素等生长因子对多肽表达的影响 这些细胞。 生长因子IGF-1（胰岛素样生长 因子-1）和TGF-α（转化生长因子α）是 能够引发许多生长刺激反应， 当应用于MCF-7人乳腺肿瘤细胞时， 线 MCF-7、MCF-7（gpt）之间的初始基线研究 和MCF-7（ras）已经发现有几种多肽 仅在MCF-7（14）、MCF-7（gpt）（5）和MCF-7（ras）（3）中表达。 细胞系之间的一些定量差异是 显然，主要的区别发生在 MCF-7 - MCF-7（gpt）和MCF-7 - MCF-7（ras）。 研究以评估 雌激素对细胞多肽表达的影响， 雌激素、IGF-1和TGF-α对细胞和 MCF-7、MCF-7（gpt）和MCF-7（ras）的分泌蛋白是 在最终分析阶段。 除此之外还有实验 使用抗雌激素LY 117018来评估对 抗雌激素多肽的表达和分泌， 耐药细胞系LY 2和MCF-7细胞。