CELL SURFACE PROTEINS AND CELLULAR ADHESION IN HEPATOCARCINOGENESIS
肝癌发生中的细胞表面蛋白和细胞粘附
基本信息
- 批准号:3963469
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:autoradiography cell adhesion cell differentiation cell growth regulation cell population study chemical carcinogenesis gel electrophoresis gene expression growth media hepatocellular carcinoma liver cells neoplasm /cancer genetics neoplastic cell oncoproteins preneoplastic state radiotracer tissue /cell culture
项目摘要
The main objective of this project is to analyze changes in homotypic
cell-to-cell adhesion during the evolution of chemically induced rat
hepatocarcinogenesis and to identify the cell surface proteins that are
involved in this process. Examination of intercellular homotypic adhesive
properties of 14 clones derived from a neonatal Fischer rat liver
epithelial cell line (FNRL) showed that the clones differ both in response
to dissociation by trypsin treatment and reaggregation rates. Increased
adhesion among the clones was associated with an increased proportion of
aneuploid cells in the clones. The parent cell line and the clones were
unable to grow in soft agarose in the absence or presence of 2 ng/ml of
epidermal growth factor (EGF). The rat hepatoma cell line H4-II-E showed
negligible capacity to reaggregate after dissociation into single cells and
these cells readily formed colonies in soft agarose. Markedly elevated
amounts of two acidic glycoproteins (105 cd and 67 kd) wre detected in the
"most adhesive" clone when the two-dimensional gel electrophoresis
(2D-PAGE) pattern of concanavalin A (Con A)-binding glycoproteins in this
clone was compared to that of the "least adhesive clone." 2D-PAGE patterns
of plasma membrane glycoproteins isolated by Con A affinity chromatography
from normal, preneoplastic and neoplastic livers showed both qualitative
and quantitative changes among the samples. Qualitative differences
consisted of four new polypeptides appearing in preneoplastic liver versus
control liver, four polypeptides lacking in neoplastic liver, and five
polypeptides appearing new in neoplastic liver compared with control
liver. These findings support the hypothesis that modulation of normal
cell surface components, especially glycoproteins involved in cell-to-cell
or cell-to-matrix adhesion and communication, may be responsible for some
of the biological behavior of cancer cells.
这个项目的主要目的是分析同型的变化
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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S S THORGEIRSSON其他文献
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{{ truncateString('S S THORGEIRSSON', 18)}}的其他基金
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大鼠 MDR 基因家族的克隆及其在正常和肿瘤肝脏中的调节
- 批准号:
3853518 - 财政年份:
- 资助金额:
-- - 项目类别:
ANALYSIS OF GENETIC ALTERATIONS DURING HEPATOCARCINOGENESIS
肝癌发生过程中的基因改变分析
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3774876 - 财政年份:
- 资助金额:
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CELLULAR AND MOLECULAR BIOLOGY OF THE HEPATIC STEM CELL COMPARTMENT
肝干细胞区室的细胞和分子生物学
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3774824 - 财政年份:
- 资助金额:
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ANALYSIS OF CELLULAR AND GENETIC ALTERATIONS DURING HEPATOCARCINOGENESIS
肝癌发生过程中的细胞和基因改变分析
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3752711 - 财政年份:
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3752778 - 财政年份:
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5201568 - 财政年份:
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