CELL SURFACE PROTEINS AND CELLULAR ADHESION IN HEPATOCARCINOGENESIS

肝癌发生中的细胞表面蛋白和细胞粘附

基本信息

项目摘要

The main objective of this project is to analyze changes in homotypic cell-to-cell adhesion during the evolution of chemically induced rat hepatocarcinogenesis and to identify the cell surface proteins that are involved in this process. Examination of intercellular homotypic adhesive properties of 14 clones derived from a neonatal Fischer rat liver epithelial cell line (FNRL) showed that the clones differ both in response to dissociation by trypsin treatment and reaggregation rates. Increased adhesion among the clones was associated with an increased proportion of aneuploid cells in the clones. The parent cell line and the clones were unable to grow in soft agarose in the absence or presence of 2 ng/ml of epidermal growth factor (EGF). The rat hepatoma cell line H4-II-E showed negligible capacity to reaggregate after dissociation into single cells and these cells readily formed colonies in soft agarose. Markedly elevated amounts of two acidic glycoproteins (105 cd and 67 kd) wre detected in the "most adhesive" clone when the two-dimensional gel electrophoresis (2D-PAGE) pattern of concanavalin A (Con A)-binding glycoproteins in this clone was compared to that of the "least adhesive clone." 2D-PAGE patterns of plasma membrane glycoproteins isolated by Con A affinity chromatography from normal, preneoplastic and neoplastic livers showed both qualitative and quantitative changes among the samples. Qualitative differences consisted of four new polypeptides appearing in preneoplastic liver versus control liver, four polypeptides lacking in neoplastic liver, and five polypeptides appearing new in neoplastic liver compared with control liver. These findings support the hypothesis that modulation of normal cell surface components, especially glycoproteins involved in cell-to-cell or cell-to-matrix adhesion and communication, may be responsible for some of the biological behavior of cancer cells.
这个项目的主要目的是分析同型的变化

项目成果

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S S THORGEIRSSON其他文献

S S THORGEIRSSON的其他文献

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{{ truncateString('S S THORGEIRSSON', 18)}}的其他基金

CLONING OF THE RAT MDR GENE FAMILY AND REGULATION IN NORMAL AND NEOPLASTIC LIVER
大鼠 MDR 基因家族的克隆及其在正常和肿瘤肝脏中的调节
  • 批准号:
    3853518
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
HEPATIC STEM CELL COMPARTMENT AND LIVER TUMORS
肝干细胞区室和肝脏肿瘤
  • 批准号:
    3874676
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
ANALYSIS OF GENETIC ALTERATIONS DURING HEPATOCARCINOGENESIS
肝癌发生过程中的基因改变分析
  • 批准号:
    3774876
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
CELLULAR AND MOLECULAR BIOLOGY OF THE HEPATIC STEM CELL COMPARTMENT
肝干细胞区室的细胞和分子生物学
  • 批准号:
    3774824
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
ANALYSIS OF CELLULAR AND GENETIC ALTERATIONS DURING HEPATOCARCINOGENESIS
肝癌发生过程中的细胞和基因改变分析
  • 批准号:
    3752711
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
MULTIDRUG RESISTANCE AND PROGRAMMED CELL DEATH IN TUMORIGENESIS
肿瘤发生中的多药耐药性和程序性细胞死亡
  • 批准号:
    3752778
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
MITOGEN MEDIATED SIGNAL TRANSDUCTION IN CARCINOGENESIS
致癌过程中丝裂原介导的信号转导
  • 批准号:
    3752738
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
TRANSGENIC MODELS--COOPERATION OF C MYC AND GROWTH FACTORS IN TUMORIGENESIS
转基因模型--C MYC和生长因子在肿瘤发生中的合作
  • 批准号:
    5201568
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
POLYPEPTIDE MODULATION IN MCF-7 CELLS BY ESTROGEN AND GROWTH FACTORS
雌激素和生长因子对 MCF-7 细胞中多肽的调节
  • 批准号:
    3939733
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
GENETIC DETERMINANTS IN CHEMICAL HEPATOCARCINOGENESIS
化学性肝癌发生中的遗传决定因素
  • 批准号:
    3916835
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:

相似国自然基金

GMFG/F-actin/cell adhesion 轴驱动 EHT 在造 血干细胞生成中的作用及机制研究
  • 批准号:
    TGY24H080011
  • 批准年份:
    2024
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    0.0 万元
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Collaborative Research: NSF-BSF: How cell adhesion molecules control neuronal circuit wiring: Binding affinities, binding availability and sub-cellular localization
合作研究:NSF-BSF:细胞粘附分子如何控制神经元电路布线:结合亲和力、结合可用性和亚细胞定位
  • 批准号:
    2321481
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Collaborative Research: NSF-BSF: How cell adhesion molecules control neuronal circuit wiring: Binding affinities, binding availability and sub-cellular localization
合作研究:NSF-BSF:细胞粘附分子如何控制神经元电路布线:结合亲和力、结合可用性和亚细胞定位
  • 批准号:
    2321480
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Probing the mechano-biology of cell-cell adhesion in a novel single cell assay
在新型单细胞测定中探讨细胞间粘附的力学生物学
  • 批准号:
    EP/Y002245/1
  • 财政年份:
    2024
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    --
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    Research Grant
Coating the cell surface with adhesive polymers: a strategy to enhance cell adhesion
用粘附聚合物涂覆细胞表面:增强细胞粘附的策略
  • 批准号:
    EP/X037622/1
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Research Grant
In vivo and ex vivo lessons from somatic adrenal mutations in cell adhesion molecule 1 for physiological and pathological production of aldosterone
细胞粘附分子 1 体细胞肾上腺突变对醛固酮生理和病理产生的体内和离体教训
  • 批准号:
    MR/X018970/1
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
    Fellowship
Role of the Glycocalyx and Spike-Like Proteins in Virus-Cell Adhesion
糖萼和刺突状蛋白在病毒-细胞粘附中的作用
  • 批准号:
    2226779
  • 财政年份:
    2023
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    --
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    Standard Grant
Molecular mechanism for the regulation of neuroblast chain migration by the cell adhesion molecule.
细胞粘附分子调节神经母细胞链迁移的分子机制。
  • 批准号:
    23K05770
  • 财政年份:
    2023
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    --
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    Grant-in-Aid for Scientific Research (C)
Study of dynamic three-dimensional structure of pathogenic bacterial pili and its host cell adhesion mechanism by X-ray structure and cryo-EM
X射线结构和冷冻电镜研究病原菌菌毛动态三维结构及其宿主细胞粘附机制
  • 批准号:
    23K04944
  • 财政年份:
    2023
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    --
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Non-Canonical Roles for Cell-Adhesion Molecules in Presynaptic Assembly
细胞粘附分子在突触前组装中的非典型作用
  • 批准号:
    10751904
  • 财政年份:
    2023
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    --
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Cell-adhesion mechanisms of inhibitory synapse specificity and their dysfunction in neuropsychiatric disorders
抑制性突触特异性的细胞粘附机制及其在神经精神疾病中的功能障碍
  • 批准号:
    488087
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
    Operating Grants
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