POLYPEPTIDE MODULATION IN MCF-7 CELLS BY ESTROGEN AND GROWTH FACTORS
雌激素和生长因子对 MCF-7 细胞中多肽的调节
基本信息
- 批准号:3916861
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
The purpose of this project was to utilize the established human
mammary tumor cell lines (MCF-7, MCF-7gpt (produced by transfection
with the Eco-gpt selectable gene marker), MCF-7ras (produced by
transfection with Eco-gpt and the v-Hras oncogene) and LY2) to
investigate the effect of antiestrogens, estrogen and other growth
factors on the polypeptide expression of both cellular and secreted
proteins in these cells. The growth factors IGF-I (insulin like
growth factor-I) and TGF-alpha (transforming growth factor alpha)
are able to elicit many of the growth stimulating responses of
estrogen when applied to the MCF-7 human mammary tumor cell line.
Analysis of the 2-dimensional polyacrylamide gels from cellular
proteins of the MCF-7gpt and MCF-7ras cells which have been treated
with 17-beta-estradiol or an ethanol vehicle showed 12 polypeptides
which were consistently altered. Of these, four polypeptides were
clearly affected the same way by ras transfection and estrogen
stimulation. Sixteen secreted polypeptides were altered by at
least twofold subsequent to either estrogen or v-Hras. Opposing
effects of estrogen and v-Hras were observed for a number of
polypeptides, but synergistic effects of v-Hras and estrogen were
seen for three polypeptides detected by autoradiography. Studies
to ascertain the effects of the growth factors IGF-I and TGF-alpha
on the expression and secretion of these polypeptides are in the
final stages of analysis. Additional to this are experiments using
the anti-estrogen LY117018 to assess the effect on polypeptide
expression and secretion from the antiestrogen-resistant cell line
LY2 and the MCF-7 cells.
该项目的目的是利用现有的人类
乳腺肿瘤细胞系(MCF-7,MCF-7 gpt(通过转染产生
用Eco-gpt可选择基因标记)、MCF-7 ras(由
用Eco-gpt和v-Hras癌基因转染)和LY 2),
研究抗雌激素、雌激素和其他生长激素的作用
细胞和分泌的多肽表达的影响因素
这些细胞中的蛋白质。 生长因子IGF-I(胰岛素样
生长因子-I)和TGF-α(转化生长因子α)
能够引发许多生长刺激反应,
当应用于MCF-7人乳腺肿瘤细胞系时,雌激素。
细胞培养中的二维聚丙烯酰胺凝胶分析
已处理的MCF-7 gpt和MCF-7 ras细胞的蛋白质
与17-β-雌二醇或乙醇载体的共表达显示出12种多肽
一直在改变 其中,四种多肽是
ras基因转染和雌激素也以同样的方式影响
刺激. 16种分泌多肽被at
雌激素或v-Hras后至少两倍。 相对
雌激素和v-Hras的影响被观察到,
多肽,但v-Hras和雌激素的协同作用,
通过放射自显影术检测的三种多肽可见。 研究
为了确定生长因子IGF-I和TGF-α的作用,
对这些多肽的表达和分泌的影响在
分析的最后阶段。 除此之外,还有使用
抗雌激素LY 117018以评估对多肽的影响
抗雌激素抗性细胞系的表达和分泌
LY 2和MCF-7细胞。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('S S THORGEIRSSON', 18)}}的其他基金
ANALYSIS OF GENETIC ALTERATIONS DURING HEPATOCARCINOGENESIS
肝癌发生过程中的基因改变分析
- 批准号:
3774876 - 财政年份:
- 资助金额:
-- - 项目类别:
CLONING OF THE RAT MDR GENE FAMILY AND REGULATION IN NORMAL AND NEOPLASTIC LIVER
大鼠 MDR 基因家族的克隆及其在正常和肿瘤肝脏中的调节
- 批准号:
3853518 - 财政年份:
- 资助金额:
-- - 项目类别:
ANALYSIS OF CELLULAR AND GENETIC ALTERATIONS DURING HEPATOCARCINOGENESIS
肝癌发生过程中的细胞和基因改变分析
- 批准号:
3752711 - 财政年份:
- 资助金额:
-- - 项目类别:
MULTIDRUG RESISTANCE AND PROGRAMMED CELL DEATH IN TUMORIGENESIS
肿瘤发生中的多药耐药性和程序性细胞死亡
- 批准号:
3752778 - 财政年份:
- 资助金额:
-- - 项目类别:
CELL SURFACE PROTEINS AND CELLULAR ADHESION IN HEPATOCARCINOGENESIS
肝癌发生中的细胞表面蛋白和细胞粘附
- 批准号:
3963469 - 财政年份:
- 资助金额:
-- - 项目类别:
TRANSGENIC MODELS--COOPERATION OF C MYC AND GROWTH FACTORS IN TUMORIGENESIS
转基因模型--C MYC和生长因子在肿瘤发生中的合作
- 批准号:
5201568 - 财政年份:
- 资助金额:
-- - 项目类别:
CELLULAR AND MOLECULAR BIOLOGY OF THE HEPATIC STEM CELL COMPARTMENT
肝干细胞区室的细胞和分子生物学
- 批准号:
3774824 - 财政年份:
- 资助金额:
-- - 项目类别:
POLYPEPTIDE MODULATION IN MCF-7 CELLS BY ESTROGEN AND GROWTH FACTORS
雌激素和生长因子对 MCF-7 细胞中多肽的调节
- 批准号:
3939733 - 财政年份:
- 资助金额:
-- - 项目类别:
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