RESPONSE OF MAMMALIAN CELLS TO HALOGENATED PYRIMIDINES

当某些卤代嘧啶如溴脱氧尿苷 （BrdUrd）和碘脱氧尿苷（IdUrd）被掺入到 细胞DNA，细胞变得更加敏感，电离 辐射 这一观察结果导致了几项临床研究 多年来和最近在NCI评估是否 IdUrd可实现对肿瘤的选择性增敏 注射后进行放疗。 一个重要的问题出现在 这些关于药物是否真的 整合到细胞中。 本研究旨在获得 关于这个问题的信息通过使用：a）一个IdUrd 单克隆抗体和HPLC测定，以实际定量 与正常组织相比，肿瘤中IdUrd的量; B）IdUrd 单克隆抗体流式细胞仪分选S期细胞 并确定人肿瘤的标记指数;和c）评价 IdUrd可能增强化疗药物的作用， 合并。 进一步的研究对低血糖的作用提出了质疑。 用卤代嘧啶进行剂量率辐照。

When certain halogenated pyrimidines such as bromodeoxyuridine (BrdUrd) and iododeoxyuridine (IdUrd) are incorporated into cellular DNA, the cells become more sensitive to ionizing radiation. This observation has led to several clinical studies over the years and recently at the NCI to evaluate whether selective sensitization of tumors could be achieved by IdUrd infusion followed by radiation. An important question arises in these studies regarding whether or not the drug actually is incorporated into cells. This study proposes to obtain information regarding this question by using: a) a IdUrd monoclonal antibody and HPLC assays to actually quantitate the amount of IdUrd in tumor compared to normal tissue; b) IdUrd monoclonal antibody to sort out S phase cells by flow cytometry and determine labelling index of human tumors; and c) evaluation of the possible potentiation of chemotherapy drugs by IdUrd incorporation. Further studies have questioned the role of low dose rate irradiation with halogenated pyrimidines.