CHROMOSOME ALTERATIONS AND PROTO-ONCOGENES TRANSPOSITION IN CARCINOGENESIS

致癌过程中的染色体改变和原癌基因转座

• 批准号: 3939732
• 负责人: N C POPESCU
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939732
• 关键词: B lymphocyte; Burkitt's lymphoma; breast neoplasms; carcinogenesis; cellular oncology; chromosome disorders; chromosome translocation; cytochrome P450; genetic mapping; human tissue; lymphocytic leukemia; neoplasm /cancer genetics; neoplastic growth; oncogenes; point mutation

Two newly isolated proto-oncogenes, erbB-2 and arg have been localized by in situ hybridization to chromosomes 17 and 1, respectively. The erbB-2 gene is frequently overexpressed in mammary cancer and the long arm of chromosome 1, where arg gene is located, is involved in structural rearrangements or duplications in the majority of the solid tumors. Human cytochrome P1-450 was localized on chromosome 15 at the site of breakpoint of the translocation 15;17 characteristic for acute promyelocytic leukemia. A North American Burkitt's lymphoma cell line exhibits, in addition to a common 8;22 reciprocal translocation, two other translocations occurring near proto- oncogene sites; however, only myc mRNA is highly elevated, indicating that myc gene was implicated in neoplastic development of this B-cell malignancy.

两个新分离的原癌基因erbB-2和arg已经被发现， 通过原位杂交定位于染色体17和1， 分别 erbB-2基因经常在乳腺癌中过度表达， 乳腺癌和1号染色体长臂，其中arg 基因被定位，参与结构重排或 在大多数实体瘤中是重复的。 人类 细胞色素P1-450定位于15号染色体上， 易位的断点15;17特征急性 早幼粒细胞白血病 一种北美伯基特淋巴瘤 细胞系显示，除了一个共同的8;22倒数 易位，另外两个易位发生在原- 癌基因位点;然而，只有myc mRNA高度升高， 提示myc基因与肿瘤的发生有关， B细胞恶性肿瘤的发展