CHROMOSOME ALTERATIONS AND PROTO-ONCOGENES TRANSPOSITION IN CARCINOGENESIS

致癌过程中的染色体改变和原癌基因转座

• 批准号: 3916860
• 负责人: N C POPESCU
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916860
• 关键词: Burkitt's lymphoma; Papillomavirus; breast neoplasms; carcinogenesis; chromosome disorders; chromosome translocation; cytochrome P450; genetic mapping; hormone binding protein; human tissue; in situ hybridization; myelogenous leukemia; neoplasm /cancer genetics; nucleic acid hybridization; oncogenes; protooncogene; sex chromosomes; thyroid hormones; virus DNA

Fyn, a novel human proto-oncogene of the src gene family, has been localized by in situ hybridization on chromosome 6. This localization shows that src genes are located on different chromosomes and represent distinct loci. Another proto-oncogene, dbl. isolated from a diffuse B-cell lymphoma, was localized on the X chromosome. Sex chromosomes, therefore, like autosomes, have oncogenic potential. A gene coding for a human cellular thyroid hormone binding protein (P55) was assigned on chromosome 17. The precise localization of these three genes will permit the evaluation of possible effects of chromosome changes on their structure and activity in malignancies and chromosome syndromes. Cellular sequences flanking an integrated human papillomavirus (HPV)-18 DNA copy was localized on chromosomes from normal and HeLa cells to chromosome 8 near the myc gene. The integration at this site was accompanied by amplification of viral sequences and increased myc RNA expression. These alterations may have contributed to the development of this malignancy.

Fyn是一种新的人类原癌基因，属于src基因家族。 通过6号染色体上的原位杂交定位。这 定位表明src基因位于不同的区域。 染色体，代表不同的基因座。另一个原癌基因， DBL.从弥漫性B细胞淋巴瘤中分离出来，定位于 X染色体。因此，性染色体就像常染色体一样，有 致癌潜力。编码人类细胞甲状腺的基因 激素结合蛋白(P55)定位于17号染色体。 这三个基因的精确定位将使 染色体变化对其可能影响的评估 恶性肿瘤和染色体综合征的结构和活性。 整合型人乳头瘤病毒两侧的细胞序列 (HPV)-18DNA拷贝定位于正常和HeLa的染色体上 8号染色体靠近myc基因的细胞。在这方面的整合 位点伴随着病毒序列的扩增和 Myc RNA表达增加。这些变化可能会 促成了这种恶性疾病的发展。