FLUOROIMMUNOASSAY APPARATUS

具有衰减时间常数的新型稀土络合荧光探针 远长于背景衰减时间常数的典型。 有机材料提供了极大的改进潜力 时间分辨荧光法的灵敏度。然而，如果他们要 竞争对手的放射免疫分析方法的灵敏度要求成为 所以荧光检测器的过载恢复是如此之大 大问题。过去关于这个问题的大量工作在很大程度上 不适用于非常低的速度和级别要求 情况。因此，最初的努力正在应用于 确定最佳荧光检测装置及辅助装置 信号过载限制电路。与之相关的问题 在极低样品浓度下的荧光探针 与疾控中心合作研究，疾控中心对此感兴趣 作为艾滋病研究的另一种工具。最后是一个评价 有关激励、光学滤波和数字信号的详细信息 将制定和优化工艺。

New rare earth chelate fluorescent probes with decay time constants much longer than the background decay time-constant of typical. organic materials offer the potential of a great improvement in sensitivity of time-resolved fluorimetry. However, if they are to rival radioimmunoassay methods the sensitivity requirement becomes so great that overload recovery of the fluorescence detector is a major problem. Extensive past work on this problem is largely inapplicable to the very low speed and level requirements of this situation. Consequently initial effort is being applied to determining the optimum fluorescence detection device and ancillary signal overload limiting circuitry. Problems related to the fluorescent probe at extremely low sample concentrations will be studied in collaboration with CDC, which is interested in this approach as another tool for AIDS research. Finally an evaluation of the details of excitation, optical filtering, and digital signal processing will be worked out and optimized.