FLUOROIMMUNOASSAY APPARATUS

新型稀土螯合荧光探针的衰减时间常数远大于 比典型有机材料的背景衰减时间常数长 在时间分辨的灵敏度方面提供了极大的改进的潜力， 荧光测定法 然而，为了与放射免疫测定方法竞争， 灵敏度的改善是如此之大， 荧光检测器是一个主要问题。 过去在这方面的大量工作 这个问题在很大程度上不适用于非常低的速度和水平 这种情况的要求。 因此，初步努力正在进行中。 应用于确定最佳荧光检测装置， 辅助信号过载限制电路。 相关问题 在极低的样品浓度下的荧光探针将在 与CDC合作，CDC对这种方法感兴趣， 艾滋病研究的工具。 最后，评价了 激发，光学滤波和数字信号处理将工作 out和优化。

New rare earth chelate fluorescent probes with a decay time constant much longer than the background decay time-constant of typical organic materials offer the potential of a great improvement in sensitivity of time resolved fluorimetry. However, to rival radioimmunoassay methods the required sensitivity improvement is so great that overload recovery of the fluorescence detector is a major problem. Extensive past work on this problem is largely inapplicable to the very low speed and level requirements of this situation. Consequently initial effort is being applied to determining the optimum fluorescence detection device and ancillary signal overload limiting circuitry. Problems related to the fluorescent probe at extremely low sample concentrations will be studied in collaboration with CDC, which is interested in this approach as another tool for AIDS research. Finally an evaluation of the details of excitation, optical filtering, and digital signal processing will be worked out and optimized.