FLUOROIMMUNOASSAY APPARATUS

具有衰减时间的新型稀土螯合荧光探针 比背景衰减时间常数长得多的常数 典型的有机材料提供了巨大的潜力， 提高时间分辨荧光法灵敏度。 然而，如果他们是竞争对手的放射免疫测定方法， 灵敏度要求变得如此之大， 荧光检测器是一个主要问题。 广泛的过去 在这个问题上的工作在很大程度上不适用于非常低的速度 这种情况的要求。 因此，初始 正在努力确定最佳荧光 检测装置和辅助信号过载限制电路。 在极低温度下与荧光探针相关的问题 将与CDC合作研究样本浓度， which哪一个is interested兴趣in this approach方法as another另一个tool工具for AIDS艾滋病 research. 最后，评价激励的细节， 光学滤波和数字信号处理 并优化。

New rare earth chelate fluorescent probes with decay time constants much longer than the background decay time-constant of typical organic materials offer the potential of a great improvement in sensitivity of time resolved fluorimetry. However, if they are to rival radioimmunoassay methods the sensitivity requirement becomes so great that overload recovery of the fluorescence detector is a major problem. Extensive past work on this problem is largely inapplicable to the very low speed and level requirements of this situation. Consequently initial effort is being applied to determining the optimum fluorescence detection device and ancillary signal overload limiting circuitry. Problems related to the fluorescent probe at extremely low sample concentrations will be studied in collaboration with CDC, which is interested in this approach as another tool for AIDS research. Finally, an evaluation of the details of excitation, optical filtering, and digital signal processing will be worked out and optimized.