GENE REGULATION WITHIN THE NERVOUS SYSTEM

我们接近了细胞特异性的和发育调节的 利用神经元在神经系统内表达蛋白质 以特异性(NSEL)和非神经元(NNE)烯醇酶同工酶为模型。 构建人脑cDNA库和基因组DNA文库 这些和其他大脑特定蛋白的基因可能是 与世隔绝的，有特点的。同时使用抗体和 人类NSE和NNE的寡核苷酸探针、cDNA已经被 分离并测序。利用这些cdna的独特区域 克隆作为探针，发育和细胞特异性调节 这些蛋白质中每一种的mrna的出现都可以被研究。 采用原位杂交的方法。人类每一个染色体的基因座 这些同工酶中的一种将被鉴定。此外，隔离 每一种蛋白质的人类基因组克隆应该提供 神经元和神经胶质细胞表达调控的研究进展 人类神经细胞分化过程中的特异蛋白 系统处于正常和疾病状态。NSE的正常特异性 神经来源的细胞系和特定DNA的可用性 针对NSE的探测应提供一种有用的方法 神经源性正常细胞和肿瘤细胞谱系的特征。

We approached the cell-specific and developmentally regulated expression of proteins within the nervous system using the neuron specific (NSEl and non-neuronal (NNE) enolase isozymes as a model. Human brain cDNA and genomic DNA libraries were constructed so that the genes for these and other brain specific proteins could be isolated and characterized. Using both antibodies and oligonucleotide probes, cDNAs for both human NSE and NNE have been isolated and sequenced. Employing unique regions of these cDNA clones as probes, the developmentally and cell-specific regulated appearance of mRNA for each of these proteins can be investigated using in-situ hybridization. The human chromosome loci for each of these isozymes will be identified. In addition, the isolation of human genomic clones for each of these proteins should provide information on the regulation of expression of neuron and glial specific proteins during cell differentiation of the human nervous system in normal and disease states. The normal specificity of NSE for neural derived cell lines and the availability of specific DNA probes for NSE should provide a useful approach to the characterization of neural derived normal and tumor cell lineages.