GENE REGULATION WITHIN THE NERVOUS SYSTEM

我们研究了细胞特异性和发育调控的 使用神经元在神经系统内表达蛋白质 特异性（NSE 1）和非神经元（NNE）烯醇化酶同工酶作为模型。 构建人脑cDNA和基因组DNA文库， 这些和其他大脑特异性蛋白质的基因可能是 分离并表征。 使用抗体和 作为寡核苷酸探针，人NSE和NNE的cDNA已经被 分离并测序。 利用这些cDNA的独特区域 克隆作为探针，发育和细胞特异性调节 可以研究这些蛋白质中的每一种的mRNA的出现 使用原位杂交。 每个人的染色体位点 将鉴定这些同工酶。 此外，隔离 这些蛋白质的人类基因组克隆应该提供 神经元和胶质细胞表达调控的信息 人神经细胞分化过程中的特异蛋白 系统处于正常和疾病状态。 NSE的正常特异性 神经源性细胞系和特异性DNA的可用性 NSE探针应该提供一种有用的方法， 神经衍生的正常和肿瘤细胞谱系的表征。

We approached the cell-specific and developmentally regulated expression of proteins within the nervous system using the neuron specific (NSEl and non-neuronal (NNE) enolase isozymes as a model. Human brain cDNA and genomic DNA libraries were constructed so that the genes for these and other brain specific proteins could be isolated and characterized. Using both antibodies and oligonucleotide probes, cDNAs for both human NSE and NNE have been isolated and sequenced. Employing unique regions of these cDNA clones as probes, the developmentally and cell-specific regulated appearance of mRNA for each of these proteins can be investigated using in-situ hybridization. The human chromosome loci for each of these isozymes will be identified. In addition, the isolation of human genomic clones for each of these proteins should provide information on the regulation of expression of neuron and glial specific proteins during cell differentiation of the human nervous system in normal and disease states. The normal specificity of NSE for neural derived cell lines and the availability of specific DNA probes for NSE should provide a useful approach to the characterization of neural derived normal and tumor cell lineages.