GENE REGULATION WITHIN THE NERVOUS SYSTEM
GENE REGULATION WITHIN THE NERVOUS SYSTEM
批准号:
3921991
负责人:
E I GINNS
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
brain cell differentiation developmental genetics gene expression genetic library genetic mapping genetic regulation genome glia human tissue immunochemistry in situ hybridization isozymes molecular oncology neoplastic cell nervous system disorder nervous system neoplasms neurons nucleic acid probes nucleic acid sequence phosphopyruvate hydratase protein sequence protein structure
中文摘要
我们接近了细胞特异性的和发育调节的
利用神经元在神经系统内表达蛋白质
以特异性(NSEL)和非神经元(NNE)烯醇酶同工酶为模型。
构建人脑cDNA库和基因组DNA文库
这些和其他大脑特定蛋白的基因可能是
与世隔绝的,有特点的。同时使用抗体和
人类NSE和NNE的寡核苷酸探针、cDNA已经被
分离并测序。利用这些cdna的独特区域
克隆作为探针,发育和细胞特异性调节
这些蛋白质中每一种的mrna的出现都可以被研究。
采用原位杂交的方法。人类每一个染色体的基因座
这些同工酶中的一种将被鉴定。此外,隔离
每一种蛋白质的人类基因组克隆应该提供
神经元和神经胶质细胞表达调控的研究进展
人类神经细胞分化过程中的特异蛋白
系统处于正常和疾病状态。NSE的正常特异性
神经来源的细胞系和特定DNA的可用性
针对NSE的探测应提供一种有用的方法
神经源性正常细胞和肿瘤细胞谱系的特征。
英文摘要
We approached the cell-specific and developmentally regulated
expression of proteins within the nervous system using the neuron
specific (NSEl and non-neuronal (NNE) enolase isozymes as a model.
Human brain cDNA and genomic DNA libraries were constructed so that
the genes for these and other brain specific proteins could be
isolated and characterized. Using both antibodies and
oligonucleotide probes, cDNAs for both human NSE and NNE have been
isolated and sequenced. Employing unique regions of these cDNA
clones as probes, the developmentally and cell-specific regulated
appearance of mRNA for each of these proteins can be investigated
using in-situ hybridization. The human chromosome loci for each
of these isozymes will be identified. In addition, the isolation
of human genomic clones for each of these proteins should provide
information on the regulation of expression of neuron and glial
specific proteins during cell differentiation of the human nervous
system in normal and disease states. The normal specificity of NSE
for neural derived cell lines and the availability of specific DNA
probes for NSE should provide a useful approach to the
characterization of neural derived normal and tumor cell lineages.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
MOLECULAR GENETICS OF LYSOSOMAL DISORDERS
-
批准号:3969060
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
MOLECULAR GENETIC STUDIES OF THE MUCOPOLYSACCHARIDOSES
-
批准号:3969059
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
TRANSGENIC ANIMAL MODELS OF HUMAN INHERITED DISORDERS
-
批准号:6162911
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
STUDIES OF GAUCHER DISEASE AND OTHER NEUROGENETIC DISORDERS TOWARD GENE THERAPY
-
批准号:3845237
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
SEARCH FOR DNA MARKERS LINKED TO MANIC DEPRESSIVE ILLNESS IN THE OLD ORDER AMISH
-
批准号:3845403
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
CORRECTION OF INHERITED PROTEIN DEFICIENCEIS BY GENE THERAPY
-
批准号:2578718
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
MOLECULAR GENETICS OF INHERITED NEUROLOGIC AND PSYCHIATRIC DISORDERS
-
批准号:2578719
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
MOLECULAR GENETICS OF INHERITED NEUROLOGIC AND PSYCHIATRIC DISORDERS
-
批准号:3880914
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
APPLICATION OF GENE TRANSFER TO CORRECT INHERITED ENZYME DEFICIENCIES
-
批准号:4696966
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
APPLICATION OF GENE TRANSFER TO CORRECT INHERITED ENZYME DEFICIENCIES
-
批准号:3969044
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
STUDIES OF GAUCHER DISEASE AND OTHER NEUROGENETIC DISORDERS
-
批准号:3968626
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
MAPPING FUNCTIONAL DOMAINS OF LYSOSOMAL ENZYMES
-
批准号:3969043
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
TRANSGENIC ANIMAL MODELS OF HUMAN INHERITED DISORDERS
-
批准号:3781520
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
CORRECTION OF INHERITED PROTEIN DEFICIENCIES BY GENE THERAPY
-
批准号:3859899
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
STUDIES OF GAUCHER DISEASE AND OTHER NEUROGENETIC DISORDERS
-
批准号:3880911
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
GENE REGULATION WITHIN THE NERVOUS SYSTEM
-
批准号:3968628
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
GENE REGULATION WITHIN THE NERVOUS SYSTEM
-
批准号:3880913
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
MOLECULAR GENETICS OF LYSOSOMAL DISORDERS
-
批准号:4696982
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
MOLECULAR GENETIC STUDIES OF THE MUCOPOLYSACCHARIDOSES
-
批准号:4696981
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
CORRECTION OF INHERITED PROTEIN DEFICIENCEIS BY GENE THERAPY
-
批准号:5203708
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:E I GINNS
-
依托单位:
海外基金