TRANSGENIC ANIMAL MODELS OF HUMAN INHERITED DISORDERS

人类遗传性疾病的转基因动物模型

• 批准号: 6162911
• 负责人: E I GINNS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6162911
• 关键词: congenital nervous system disorder; disease /disorder model; embryonic stem cell; gene induction /repression; gene mutation; gene targeting; genetic disorder; genetic promoter element; genetically modified animals; laboratory mouse; model design /development

In many cases naturally occurring animal models of human inherited disorders, especially those affecting the nervous system, have not been described. In those cases where the gene defect has been identified, the introduction of mutations into the germ line of mice can provide a unique opportunity to generate mice having appropriate phenotypes where disease pathogenesis and treatment can be studied. Transgenic mice are generated by both pronuclear microinjection of DNA into fertilized eggs and by gene targeting by introduction of genetically modified murine embryonic stem cells into blastocyst stage mouse embryos. In addition to the generation of "null allele" or "knockout" mice, other techniques are being developed for the introduction of subtle mutations into the germline of mice. Transgenic mice with genes under the control of cell(including glia and neuronal) or tissue specific inducible/repressible promoters are also under study, particularly with respect to gene expression in the nervous system. Murine models of inherited disorders affecting the nervous system are being produced using these techniques, and the developmental and tissue specific expression of genes is characterized in these transgenic mice. Our collaborative efforts to generate transgenic mouse models of human disorders are providing valuable tools extending our understanding of disorders affecting the nervous system and for the evaluation of new treatments, including protein/enzyme replacement, cellular transplantation, and gene transfer therapies.

在许多情况下，自然发生的动物模型的人类遗传