DIFFERENTIATION AND REGULATION OF GENE EXPRESSION IN ASTROCYTES AND NEURONS

我们已经研究了典型的蛋白质基因的调控 存在于星形胶质细胞或神经元中。 谷氨酰胺合成酶（GS）mRNA 早在E14就在大鼠中产生，然后随着 皮质类固醇的产生。 差距-43蛋白通常存在于 神经生长锥显然暴露于神经元细胞 表面，因为它可以用碘标记，被发现是一个小的 星形胶质细胞的程度，并可能涉及星形胶质细胞-神经元 互动 参与这种相互作用的蛋白质 层粘连蛋白分泌到细胞外基质中， 星形胶质细胞，并作为神经突生长因子，为PC 12 细胞和初级小脑和皮质神经元。 神经胶质 胶质酸性蛋白（GFAP）在某些细胞中可以被诱导 被剥夺了鸟嘌呤 GS、GFAP和S- 100个蛋白质被分离，启动子区域将被分离。 连接到一个报告基因，以了解诱导 这些蛋白质，以及为什么它们特别是 在星形胶质细胞中表达。 毒蕈碱的五种mRNA 乙酰胆碱受体（m1-m5）已被单独转移 到A1-L细胞，其中一次仅表达一种受体。 这 能够分析受体蛋白的偶联 对不同G因子的敏感性、对百日咳毒素的敏感性、 磷酸肌醇或cAMP的产生，以及其它性质。 到 作为对实验室其他工作的补充， 分离出与谷氨酸受体有关的基因。

We have investigated the regulation of genes of proteins typically present in astrocytes or neurons. Glutamine synthetase (GS) mRNA is made in rats as early as E14 and then increases together with corticosteroids production. The GAP-43 protein typically found in nerve growth cones apparently is exposed to the neuronal cell surface because it can be labelled with iodine, is found to a small extent in astrocytes, and may be involved in astrocyte-neuron interaction. Certainly involved in this interaction is the protein laminin which is excreted into the extracellular matrix of astrocytes and serves as a neurite outgrowth factor for both PC12 cells and primary cerebellar and cortical neurons. The glial fibrillary acidic protein (GFAP) can be induced in certain cells by guanine deprivation. The genomic DNAs of GS, GFAP and the S- 100 protein are being isolated and the promoter region will be attached to a reporter gene in order to understand the induction of these proteins and the reason why they are specifically expressed in astrocytes. The five mRNA's of the muscarinic acetylcholine receptor (ml-m5) have been individually transferred to A1-L cells where only one receptor at a time is expressed. This has enabled the analysis of the coupling of the receptor protein to different G-factors, the sensitivity to pertussis toxin, the production of phosphoinositides or cAMP, and other properties. To complement other work in the laboratory, attempts are underway to isolate the genes involved with one of the glutamate receptors.