CONTROL MECHANISMS AND DIFFERENTIATION

控制机制和差异化

• 批准号: 4696780
• 负责人: E FREESE
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4696780
• 关键词: Bacillus subtilis; S adenosylmethionine; Saccharomyces; aminoacid; bacterial DNA; bacterial genetics; cell differentiation; cell wall; chromatography; cyclic AMP; deficient growth media; enzyme induction /repression; fungal genetics; gel electrophoresis; genetic library; glucose dehydrogenases; glutamate ammonia ligase; growth media; guanosine triphosphate; hydrolase; meiosis; membrane permeability; microorganism culture; microorganism growth; microorganism metabolism; molecular biology; molecular cloning; mutant; nucleic acid inhibitor; pancreatic ribonuclease; peptidoglycan; sporogenesis

The switch from mitosis to meiosis and sporulation in the yeast Saccharomyces cerevisiae, which is normally initiated by nutritional deprivation, can be specifically initiated by partial guanine or methionine deprivation. All these conditions led to the decrease of intracellular GTP whereas the concentrations of other nucleotides remain constant, increase or decrease, depending on the particular condition used to initiate meiosis. Involvement of other metabolites in sporulation induction has been ruled out; in particular, conditions can be found under which the cellular GTP decreases while that of S-adenosylmethionine or cyclic AMP does not change and yet the cells go through meiosis and sporulate well. Nuclear and nuclear matrix proteins where subjected to two-dimensional electrophoresis and certain proteins were found to change during meiosis. The gene for one nuclear matrix proteins was cloned from both a mouse and a yeast DNA library. The gene for glutamine synthetase was similarly obtained from these two libraries. In Bacillus subtilis partially inhibitory concentrations of ethionine caused continual sporulation in eth mutants. This effect depends on the conversion of ethionine to S-adenosylethionine and presumably involves the inhibition of a methylation reaction.

酵母菌从有丝分裂到减数分裂和产孢子的转换 酿酒酵母，它通常是由营养引发的 剥夺，可由部分鸟嘌呤或蛋氨酸特异性地启动 剥夺。所有这些条件都导致细胞内GTP的降低 而其他核苷酸的浓度保持不变，增加 或减少，这取决于用于启动的特定条件 减数分裂。其他代谢物参与孢子形成诱导 已被排除；尤其是，可以找到在以下情况下 细胞GTP降低，而S-腺苷蛋氨酸或环磷酸腺苷 没有变化，但细胞经过减数分裂，孢子形成良好。 核蛋白和核基质蛋白的二维结构 在减数分裂过程中，电泳率和某些蛋白质发生了变化。 一个核基质蛋白的基因是从一只小鼠和一只 酵母DNA文库。谷氨酰胺合成酶的基因也类似。 从这两个库中获取。枯草芽孢杆菌部分 乙硫氨酸抑制浓度引起ETH连续产孢量 变种人。这一效应取决于乙硫氨酸转化为 S-腺苷硫氨酸，推测涉及抑制甲基化 反应。