CONTROL MECHANISMS AND DIFFERENTIATION

控制机制和差异化

• 批准号: 3968882
• 负责人: E FREESE
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3968882
• 关键词: Bacillus subtilis; DNA; Saccharomyces; bacterial DNA; bacterial genetics; carbohydrate biosynthesis; cell differentiation; cell wall; chromatography; cyclic AMP; deficient growth media; enzyme induction /repression; fungal genetics; gel electrophoresis; genetic library; genetic manipulation; growth media; guanosine triphosphate; meiosis; membrane permeability; microorganism culture; microorganism growth; microorganism metabolism; molecular biology; molecular cloning; mutant; nucleic acid inhibitor; pancreatic ribonuclease; sporogenesis

This report is the last one concerning work on the induction of mitosis and meiosis in the yeast Saccharomyces cerevisiae and the characterization of nuclear proteins in yeast and mouse cells. Previously, we had found that meiosis is initiated by the specific decrease of GTP. We have now shown that this initiation does not require a change in the concentration of cAMP and that GTP deprivation also causes an increase in the synthesis of glycogen. cAMP also does not mediate catabolite repression. But the presence of a carbon source is required for the normal completion of spores. Since the RASval gene prevents sporulation of yeast during nitrogen starvation, we have constructed a gua RASval double mutant; it cannot sporulate or produce glycogen during guanine deprivation which shows that the RASval mutation is always dominant. We have also characterized nuclear matrix proteins and isolated the cDNA gene for one of them from a mouse brain cDNA library.

这是关于诱导有丝分裂和 酿酒酵母减数分裂及其特性的研究 酵母和小鼠细胞中的核蛋白。此前，我们发现， 减数分裂是由GTP的特异性降低启动的。我们现在已经展示了 这个启蒙不需要改变cAMP的浓度 而GTP的剥夺也会导致合成的增加 糖原。CAMP也不调节分解代谢抑制。但是 碳源的存在是正常完成的必要条件 孢子。由于RASval基因可防止酵母菌在 氮饥饿时，我们构建了一个gua rasval双重突变体；它 在鸟嘌呤剥夺过程中不能产生孢子或产生糖原，这表明 拉斯瓦尔突变总是占主导地位。我们还描述了 并分离了其中一种核基质蛋白的cdna基因。 小鼠脑c DNA文库。