PURCHASE OF A CIRCULAR DICHROISM SPECTROPHOTOMETER

购买圆二色分光光度计

• 批准号: 3519872
• 负责人: JOHN H DAWSON
• 金额: $ 11.1万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-18 至 1989-04-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519872
• 关键词: biomedical equipment purchase; circular dichroism; site directed mutagenesis; spectrometry

Funds are requested for the purchase of a state of the art circular dichroism spectrophotometer with accessories for stopped flow and low temperature experiments and with a 15 kilogauss electromagnet to enable magnetic circular dichroism to be carried out. The new instrument will replace a thirteen year old model that has become obsolete and unreliable. The new instrument is twenty times more sensitive than the old model and is configured for simultaneous measurement of UV-visible absorption and circular dichroism spectra. The new instrument will also be equipped to make measurements from 175 to 1050 nm, a much expanded spectral window from that currently available. The specific areas of research of the primary users will focus on the use of the new instrument for the following purposes: (a) the continued development of magnetic circular dichroism spectroscopy as a probe of heme iron coordination structure and the initial examination of the use of this technique to study chlorin iron systems, (b) the application of circular dichroism spectroscopy to investigate and quantify the allosteric interconversion of the R and T states of chemically modified hemoglobins, (c) the use of stopped flow circular dichroism spectroscopy to study the mechanism of action of native thymidylate synthase and the use of circular dichroism spectroscopy to characterize selected mutant enzymes generated by site directed mutagenesis, and (d) the investigation of the circular dichroism properties of selenium containing biomolecules, particularly seleno-sulfides and di-selenides, in order to evaluate the potential utility of the method as a probe of the structure of selenobiomolecules.

请拨资金购买一份最先进的通告

项目成果

Notomastus lobatus chloroperoxidase and Amphitrite ornata dehaloperoxidase both contain histidine as their proximal heme iron ligand.

Notomastus lobatus 氯过氧化物酶和 Amphitrite ornata 脱卤过氧化物酶均含有组氨酸作为其近端血红素铁配体。

• DOI: 10.1021/bi9621371
• 发表时间: 1997
• 期刊: Biochemistry.
• 作者: Roach,MP;Chen,YP;Woodin,SA;Lincoln,DE;Lovell,CR;Dawson,JH
• 通讯作者: Dawson,JH

Magnetic circular dichroism spectroscopy as a probe of axial heme ligand replacement in semisynthetic mutants of cytochrome c.

磁性圆二色光谱作为细胞色素 c 半合成突变体中轴向血红素配体替代的探针。

• DOI: 10.1016/0014-5793(91)81222-t
• 发表时间: 1991
• 期刊: FEBS letters
• 影响因子: 3.5
• 作者: Rux,JJ;Dawson,JH
• 通讯作者: Dawson,JH

The H93G myoglobin cavity mutant as a versatile template for modeling heme proteins: ferrous, ferric, and ferryl mixed-ligand complexes with imidazole in the cavity.

• DOI: 10.1021/ic0007198
• 发表时间: 2000-12
• 期刊: Inorganic chemistry
• 影响因子: 4.6
• 作者: A. Pond;M. Roach;M. R. Thomas;S. Boxer;J. Dawson