GC-MS ANALYSIS OF PCDF BLOOD LEVELS IN CHILDREN EXPOSED IN VITRO

体外暴露儿童中 PCDF 血浓度的 GC-MS 分析

• 批准号: 3941545
• 负责人: W ROGAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3941545
• 关键词: child (0-11); environmental contamination; halobiphenyl /halotriphenyl compound; mass spectrometry; serum; toxicology

There have been two outbreaks of human poisoning by polychlorinated biphenyls (PCBs) and their thermal breakdown products; the first, in Japan in 1968, the second in Taiwan in 1979. Because PCBs are a world wide pollution problem, these episodes have been studied carefully, since they have presented the only opportunity to observe directly the toxicity of PCBs in human beings outside the workplace. Laboratory methods for the evaluation of these outbreaks were relatively unsophisticated in 1968; there has been great progress in analytical methods since. In collaboration with Taiwanese scientists, the Epidemiology Branch, NIEHS, had the opportunity to examine over 100 children who had been in utero at the time of the 1979 poisoning or afterward. These children continued to be affected, since the chemicals cannot be excreted from the mother's body. In a collaborative effort, the mass spectrometry workgroup will analyze approximately 120 serum samples for tetra-, penta- and hexachlorodibenzofurans at the part per trillion level. At the present time extraction procedures are being validated by RTI in preparation for the analysis of the samples.

已经发生了两起人类中毒事件， 多氯联苯及其热分解 产品;第一个，在日本于1968年，第二个在台湾于1979年。 由于多氯联苯是一个世界性的污染问题，这些事件 仔细研究过，因为他们提出了唯一的 直接观察多氯联苯对人体毒性的机会 工作场所之外的人。 实验室方法 对这些暴发的评估相对简单， 1968年;从那以后，分析方法有了很大的进步。 与台湾科学家合作，流行病学 NIEHS的分支有机会检查100多名儿童 在1979年中毒时还在子宫里的人， 之后。 这些儿童继续受到影响，因为 化学物质不能从母体排出。 在一项合作努力中，质谱仪将 分析大约120份血清样本的四，五， 六氯二苯并呋喃的含量为万亿分之一。 在 目前，RTI正在验证提取程序， 样品分析的准备。