GC-MS ANALYSIS OF PCDF BLOOD LEVELS IN CHILDREN EXPOSED IN VITRO

体外暴露儿童中 PCDF 血浓度的 GC-MS 分析

• 批准号: 3898117
• 负责人: W ROGAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3898117
• 关键词: air pollution; benzofurans; child (0-11); embryo /fetus toxicology; environmental contamination; environmental toxicology; halobiphenyl /halotriphenyl compound; human pregnant subject; human tissue; longitudinal animal study; mass spectrometry; serum; thermostability

There have been two outbreaks of human poisoning by polychlorinated biphenyls (PCBs) and their thermal breakdown products; the first, in Japan in 1968, the second in Taiwan in 1979. Because PCBs are a world wide pollution problem, these episodes have been studied carefully, since they have presented the only opportunity to observe directly the toxicity of PCBs in human beings outside the workplace. Laboratory methods for the evaluation of these outbreaks were relatively unsophisticated in 1968; there has been great progress in analytical methods since. In collaboration with Taiwanese scientists, the Epidemiology Branch, NIEHS, had the opportunity to examine over 100 children who had been in utero at the time of 1979 poisoning or afterward. These children continued to be affected, since the chemicals cannot be excreted from the mother's body. We have examined blood and cerumen samples for 2,3,7,8- tetrachlorodibenzofuran and hexachlorodibenzofuran using selected ion monitoring at a mass resolution of 5,000. Instrument sensitivity during these analyses was such that 50 femtograms of analyte could be detected on an absolute level with a signal to noise of ca. 20:1. Taking into account sample volume and recovery yields, the practical limits of detection in the actual samples was ca. 2.4 picogram per sample (2.4 parts per trillion). No TCDFs or HCDFs were observed in these samples. To verify these results, selected samples will be analyzed using the selected decomposition monitoring capabilities of the concept I-SQ hybrid mass spectrometer. This technique is less sensitive than the high resolution selected ion monitoring technique but it is more selective. These results are also consistent with the PCB results obtained by RTI in which no PCBs were observed.

已经发生了两起多氯化人类中毒事件 联苯及其热击穿产物；第一，在 1968年在日本，1979年在台湾排名第二。因为多氯联苯是一个世界 广泛的污染问题，这些事件一直被仔细研究，自 他们提供了直接观察毒性的唯一机会 在工作场所以外的人类体内的多氯联苯。实验室检测方法 对这些疫情的评估在#年相对简单。 1968年；自那以后，分析方法有了很大的进步。在……里面 与台湾科学家合作，NIEHS流行病学分会， 有机会检查了100多名在宫内的儿童 1979年中毒的时间或之后。这些孩子仍然是 受影响，因为这些化学物质不能从母亲的体内排泄出来。 我们已经检查了血液和脑脊液样本中的2，3，7，8- 使用选择离子的四氯二苯并呋喃和六氯二苯并呋喃 以5,000质量分辨率进行监测。仪器灵敏度在 这些分析可以检测到50毫微克的分析物 在绝对水平上，信噪比约为20：1。 考虑到样品量和回收率，实际限度 实际样品中的检测值约为每个样品2.4微微克(2.4微克 百万分之几)。在这些样品中没有观察到TCDF或HCDF。 为了验证这些结果，选定的样本将使用 Concept i-SQ混合动力车的精选分解监测能力 质谱仪。这项技术不如高灵敏度。 分辨率选择离子监测技术，但选择性更强。 这些结果也与RTI在年获得的印刷电路板结果一致 没有观察到多氯联苯。