MASS SPECTROMETRY FACILITY FOR BIOMEDICAL RESEARCH

用于生物医学研究的质谱设备

• 批准号: 3103629
• 负责人: KLAUS BIEMANN
• 金额: $ 69.57万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1974
• 资助国家: 美国
• 起止时间: 1974-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3103629
• 关键词: Reye's syndrome; aflatoxins; antineoplastics; biomedical automation; biomedical equipment resource; carcinogens; chemical information system; chemical structure function; clinical chemistry; croton oil; gas chromatography; gas chromatography mass spectrometry; laser spectrometry; mass spectrometry; membrane structure; methotrexate analog; molecular pathology; nuclear medicine; phencyclidine; radionuclide diagnosis; sphingolipids; technetium

To assure that the M.I.T. Mass Spectrometry Facility remains in the forefront of the field the area of research will be expanded to large polar molecules (up to l0,000 daltons and possibly beyond). Methods for the ionization of such molecules will be improved. Major emphasis will be on "soft" ionization techniques such as field desorption, fast atom bombardment and direct chemical ionization applied to large, polar molecules of biological interest. Laser-induced fragmentation will be investigated as a means for deriving more structural information. Proteins, oligopeptides, nucleotides, membrane structure, sphingo lipids, drug metabolites are major areas of interest. This methodology will be made available to the biomedical community through collaboration or as a service.

确保M.I.T.质谱设施仍在 该领域的最前沿研究领域将扩展到大极地 分子（最高l0,000 dalton，甚至可能是超越）。 该方法 这种分子的电离将得到改善。 主要重点将放在“软”电离技术（例如字段）上 解吸，快速原子轰击和直接化学电离应用于 生物学意义的大，极性分子。 激光诱导的碎裂 将研究作为获取更多结构信息的一种手段。 蛋白质，寡肽，核苷酸，膜结构，鞘脂脂质， 药物代谢产物是主要感兴趣的领域。 该方法将通过 协作或服务。