Reduction and refinement of murine models of bacterial infection

细菌感染小鼠模型的简化和完善

• 批准号: G0800720/1
• 负责人: Shiranee Sriskandan
• 金额: $ 35.26万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0800720%2F1
• 关键词: Reduction; refinement; murine; models; bacterial

According to estimates by the World Health Organisation, in 2002 infectious diseases caused by bacteria, viruses and parasites killed 14.7 million people worldwide ? 26% of all deaths. In the search for better vaccines and drugs, scientists often use mice to investigate how these organisms cause disease. In such studies the first signs of disease are clinical symptoms like weight loss and ruffled fur. In this research we will develop genetic tools to make a number of infectious bacteria bioluminescent and use state of the art bioluminescence imaging (BLI) technology to refine existing mouse experiments. Using very sensitive cameras we can detect the light produced by bioluminescent bacteria from within an anaesthetised animal. Multiple imaging of the same animal over the course of an experiment allows the disease progression to be followed more accurately, while using less animals. As bioluminescence is quantifiable and related to bacterial numbers, the changing bioluminescent signal can be used to estimate whether an animal will survive or die and so allows for humane euthanasia perhaps even before the appearance of clinical symptoms. We will use this technology refine our protocols and reduce the numbers of animals in experiments.

根据世界卫生组织的估计，2002年，由细菌、病毒和寄生虫引起的传染病在全世界造成1470万人死亡。占所有死亡人数的26%。在寻找更好的疫苗和药物时，科学家经常使用小鼠来研究这些生物体如何引起疾病。在这些研究中，疾病的第一个迹象是临床症状，如体重减轻和皮毛起皱。在这项研究中，我们将开发遗传工具，使一些传染性细菌生物发光，并使用最先进的生物发光成像（BLI）技术来完善现有的小鼠实验。使用非常灵敏的相机，我们可以检测到麻醉动物体内的生物发光细菌产生的光。在实验过程中对同一动物进行多次成像，可以更准确地跟踪疾病进展，同时使用更少的动物。由于生物发光是可量化的并且与细菌数量相关，因此变化的生物发光信号可以用于估计动物是否会存活或死亡，因此甚至可能在临床症状出现之前就允许人道安乐死。我们将利用这项技术改进我们的方案，减少实验中的动物数量。