AGE-RELATED CHANGES IN THE VESTIBULAR LABYRINTH

前庭迷路与年龄相关的变化

• 批准号: 3115399
• 负责人: JANIE C PARK
• 金额: $ 5.89万
• 依托单位: FLORIDA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-05-01 至 1988-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3115399
• 关键词: aging; animal age group; cell death; cell type; cellular pathology; disease /disorder model; ear hair cell; electron microscopy; genotype; hearing disorders; histology; image processing; labyrinth; microscopy; neural transmission; organelles; ototoxin; sign /symptom; synapses; vestibular apparatus; vestibular pathway

Equilibular disturbances are common in the elderly (1) and this decline in function probably indicates cellular deterioration. However, in humans it is difficult to separate the cellular changes that are age-related from those brought about by a lifetime of disease and antibiotic therapy. The establishment of a convenient animal model is important for many experiments relating balance organs and age. The aim of the proposed research is to describe the cellular changes occurring in the vestibular organs of the inner ears of C57BL/6 mice as a result of aging. Although the mouse is commonly used as a model for other types of inner ear research (2-6), no quantitative ultrastructural information is available on the aging mouse vestibular organs nor on genotype-specific progressive degeneration. The saccule, utricle, and semicircular canal ampullae of six age groups from weanling to very old will be compared to determine the temporal sequence oc cytological aging in this mouse strain. Standard morphometric and stereological techniques at the light and electron microscopic level will be used to describe the cytology of the sensory epithelium. Variation and significant changes will be analyzed at the following four levels: within an organ, among different organs in the same animal, among animals within an age group, and among the six age groups. A video image analysis system will be employed for semi-automated data collection and analysis.

平衡紊乱在老年人中很常见（1）， 功能可能表明细胞退化。 然而，在人类中， 很难将与年龄相关的细胞变化 这些都是由终生的疾病和抗生素治疗引起的。 的 建立一种方便动物模型对于许多人来说是重要的 平衡器官与年龄的相关实验 建议的目的 研究的目的是描述发生在前庭的细胞变化， C57 BL/6小鼠内耳器官的衰老。 虽然 老鼠通常被用作其他类型内耳研究的模型 (2-6)，没有定量的超微结构信息， 老化小鼠前庭器官也不对基因型特异性进行性 退化 球囊、椭圆囊和半规管壶腹 将比较从断奶到非常老的年龄组，以确定 时间序列在该小鼠品系中的细胞学老化。 标准 形态学和体视学技术， 微观水平将被用来描述感官的细胞学 上皮 变化和重大变化将在 以下四个层次：在一个机关内，在同一个机关的不同机关之间， 动物、一个年龄组内的动物和六个年龄组之间。 一 视频图像分析系统将用于半自动数据 收集和分析。