ETHANOL AND CELLULAR CALCIUM METABOLISM

乙醇和细胞钙代谢

• 批准号: 4687752
• 负责人: H C PANT
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4687752
• 关键词: alcoholic beverage consumption; alcoholism /alcohol abuse; arsenic; brain cell; calcium metabolism; early embryonic stage; erythrocyte membrane; ethanol; fluorescent dye /probe; histogenesis; mitochondrial membrane; neurotoxins; nonmammalian vertebrate embryology; radiotracer; saltwater environment; synapses; synaptosomes

Alcohol is known to affect developing tissues, evidenced by the fetal alcohol syndrome, but the molecular mechanisms of such effects are not known. In this study we have used sand dollar eggs to investigate the effects of ethanol on early stages of cell division. Video-enhanced microscopy was used to study the effects of ethanol on mitosis of sand dollar eggs. Our preliminary study showed that in the presence of low concentrations (30-50 mM) of ethanol, the immediate effects included paralysis of sperm tail motility and distortion of the embryo fertilization envelope. Later effects included disruption of cytoplasmic organization and inhibition of mitosis. The data suggest that ethanol may affect the ability of a cell to progress through the cell cycle and mitosis. Similar results were obtained when micromolar calcium chloride was injected into these eggs. The studies on sand dollar eggs could provide an experimental model for investigating the mechanism of the effects of ethanol on the early stages of development. The intracellular calcium concentration in clonal AtT-20/D16 mouse anterior pituitary tumor cells and in rat hepatocytes was measured with the fluorescent calcium indicator Quin 2. A fourfold increase in intracellular calcium was observed in 50 mM KC1 Krebs solution compared to 5 mM KC1 normal Krebs solution. In normal Krebs solution, ethanol (30 mM) caused a 10% increase in intracellular calcium concentration in rat hepatocytes, but under these conditions AtT-20/D16 cells showed no significant change. These observations suggest that the effects of ethanol on cellular calcium metabolism may vary in different cell types.

众所周知，酒精会影响发育中的组织，这一点得到了胎儿的证明 酒精综合征，但这种影响的分子机制不是 为人所知。在这项研究中，我们使用沙币鸡蛋来调查 乙醇对细胞分裂早期的影响。视频增强版 用显微镜观察乙醇对沙粒有丝分裂的影响。 一美元的鸡蛋。我们的初步研究表明，在低氧存在的情况下 浓度(30-50 mM)的乙醇，直接影响包括 精子尾部活动瘫痪与胚胎受精畸形 信封。后来的影响包括破坏细胞质组织 以及对有丝分裂的抑制。这些数据表明，乙醇可能会影响 细胞在细胞周期和有丝分裂中前进的能力。类似 当微摩尔氯化钙注射到 这些鸡蛋。对沙钱蛋的研究可以提供一种实验方法。 乙醇作用机理的研究模型 发展的早期阶段。 克隆性ATT-20/D16小鼠前角细胞内钙离子浓度的变化 大鼠垂体瘤细胞和大鼠肝细胞中 荧光钙指示剂Quin 2。细胞内增加四倍 在50 mM Kc1 Krebs溶液中观察到钙，而在5 mM Kc1溶液中观察到钙 正常的克雷布斯溶液。在正常的Krebs溶液中，乙醇(30 MM)引起 大鼠肝细胞内钙离子浓度增加10%，但 在此条件下，ATT-20/D16细胞无明显变化。 这些观察表明，乙醇对细胞内钙离子的影响 新陈代谢在不同的细胞类型中可能有所不同。