PROTEIN PHOSPHORYLATION AND SECRETION AND ETHANOL ACTIONS
PROTEIN PHOSPHORYLATION AND SECRETION AND ETHANOL ACTIONS
批准号:
3821269
负责人:
H C PANT
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
中文摘要
蛋白质的磷酸化被认为是蛋白质合成的重要途径之一。
参与细胞调控的分子机制。 的
蛋白激酶催化这些磷酸化反应。 在这
研究,与蛋白激酶活性相关的性质,
细胞骨架蛋白已在神经元和
非神经元组织。 进行了广泛的研究,
描述鱿鱼轴突神经丝激酶。 这些
研究得出结论,这种激酶是一种环状的
非核苷酸和钙依赖性蛋白激酶,
不同于酪蛋白I型和II型激酶。 轴浆
而神经丝制备物中未检测到蛋白激酶
抑制剂活性,但强抑制剂活性,这不是
透析,但热灭活,发现在神经节细胞。
这种抑制剂活性可以解释低磷酸化
星状神经节细胞中发现的活性,
鱿鱼轴突神经丝激酶活性抑制性调节
在神经节细胞体中。 在大鼠脑中,激酶活性是
与微管相关蛋白相关,
磷酸化它们。 乙醇对磷酸化的影响
微管相关蛋白(MAP 2)。
乙醇(4-24 mM)增加MAP 2的磷酸化。 在
cAMP或mM乙醇的存在,
观察到MAP 2超过对照。 更高的磷酸化
在cAMP和乙醇存在下,观察到MAP 2的增加,
MAP 2被cAMP和乙醇磷酸化总和
分开 乙醇对MAP 2影响的动力学研究
磷酸化显示磷酸化的速率增加,
MAP 2和降低Km在乙醇的存在下。 这些
研究表明,除了cAMP依赖性蛋白激酶,
蛋白激酶受乙醇和酶的影响
在MAP 2上的不同位点磷酸化。 的观察结果
乙醇对MAP 2的影响为
研究乙醇对结构和功能的影响
这些细胞骨架蛋白质和酶。
英文摘要
The phosphorylation of protein has been proposed to be one of the
molecular mechanisms involved in cellular regulation. The
protein kinases catalyze these phosphorylation reactions. In this
investigation, properties of protein kinase activity associated with
cell cytoskeletal proteins have been studied in both neuronal and
non-neuronal tissues. Extensive studies were carried out to
characterize the squid axon neurofilament kinase. These
investigations led to conclude that this kinase is a cyclic
nucleotide- and calcium-independent protein kinase which is
different from casein type I and type II kinases. The axoplasm
and neurofilament preparation had no detectable protein kinase
inhibitor activity, but strong inhibitor activity, which was not
dialyzable but was heat inactivitable, was found in ganglion cells.
This inhibitor activity may account for the low phosphorylation
activity found in the stellite ganglion cells and may indicate
inhibitory regulation of squid axon neurofilament kinase activity
in the ganglion cell bodies. In rat brain, the kinase activity is
associated with microtubule-associated proteins and
phosphorylates them. Effects of ethanol on phosphorylation of
microtubule associated protein (MAP 2) were investigated.
Ethanol (4-24 mM) increased phosphorylation of MAP 2. In the
presence of cAMP or mM ethanol, increased phosphorylation of
MAP 2 was observed over control. Much higher phosphorylation
of MAP 2 was observed in the presence of cAMP and ethanol than
the sum of phosphorylation of MAP 2 by cAMP and ethanol
separately. Kinetic studies of the influence of ethanol on MAP 2
phosphorylation reveal an increased rate of phosphorylation of
MAP 2 and a decreased Km in the presence of ethanol. These
studies suggest that protein kinase(s) other than cAMP dependent
protein kinase are influenced by ethanol and the enzyme(s)
phosphorylate at distinct sites on MAP 2. The observation that
ethanol affects MAP 2 provides an experimental basis for
investigating the effects of ethanol on the structure and function
of these cytoskeletal proteins and enzymes.
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PROTEIN PHOSPHORYLATION AND REGULATION OF CYTOSKELETON IN NEURONAL SYSTEMS
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批准号:2579565
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
PROTEIN PHOSPHORYLATION AND REGULATION OF CYTOSKELETON IN NEURONAL SYSTEMS
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批准号:3782361
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
PROTEIN PHOSPHORYLATION AND REGULATION OF CYTOSKELETON IN NEURONAL SYSTEMS
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批准号:6163030
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
PROTEIN PHOSPHORYLATION AND REGULATION OF CYTOSKELETON IN NEURONAL SYSTEMS
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批准号:5203931
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
CALCIUM METABOLISM AND PROTEIN PHOSPHORYLATION IN NEURONAL SYSTEMS
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批准号:3881776
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
ETHANOL AND MEMBRANE FUNCTION
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批准号:4687751
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
ETHANOL AND PROTEIN PHOSPHORYLATION
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批准号:4687750
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
ETHANOL AND CELLULAR CALCIUM METABOLISM
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批准号:4687752
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
ETHANOL AND NERVOUS SYSTEM DEGENERATION
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批准号:3821273
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
PROTEIN PHOSPHORYLATION AND REGULATION OF CYTOSKELETON IN NEURONAL SYSTEMS
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批准号:3760272
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
ETHANOL AND CELLULAR CALCIUM METABOLISM
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批准号:3821271
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
ETHANOL AND MEMBRANE FUNCTION
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批准号:3821270
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
ETHANOL AND NERVOUS SYSTEM DEGENERATION
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批准号:3822999
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
PROTEIN PHOSPHORYLATION AND SECRETION AND ETHANOLACTIONS
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批准号:3822995
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
BASIC CELL BIOLOGICAL MECHANISMS USING THE SQUID NERVOUS SYSTEM MODEL
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批准号:6111857
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
BASIC CELL BIOLOGICAL MECHANISMS USING THE SQUID NERVOUS SYSTEM MODEL
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批准号:2579564
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
CALCIUM METABOLISM AND PROTEIN PHOSPHORYLATION IN NEURONAL SYSTEMS
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批准号:3846236
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
ETHANOL AND NERVOUS SYSTEM DEGENERATION
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批准号:4687754
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
CALCIUM METABOLISM AND PROTEIN PHOSPHORYLATION IN NEURONAL SYSTEMS
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批准号:3922609
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
ETHANOL AND CELLULAR CALCIUM METABOLISM
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批准号:3817422
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H C PANT
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依托单位:
海外基金