ETHANOL AND MEMBRANE FUNCTION

乙醇和膜功能

• 批准号: 3821270
• 负责人: H C PANT
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3821270
• 关键词: alcoholic beverage consumption; brain cell; brain metabolism; caudate nucleus; cell membrane; ethanol; fluorescence spectrometry; fluorescent dye /probe; membrane potentials; membrane reconstitution /synthesis; neuropharmacology; neurotoxins; synaptosomes; thymus; tissue /cell culture

Ethanol is known to alter the biochemical and biophysical properties of cellular membranes. In this investigation the mechanisms of ethanol-induced membrane potential changes in brain synaptosomes and rat thymocytes were studied with fluorescence spectrophotometry using membrane potential- sensitive dyes. The effect of ethanol on membrane potential in synaptosomes was studied using rhodamine 6G fluoroscence. The fluorescence signal responds to the depolarization produced by increasing concentration of K+ outside the synaptosomes. The fluorescence intensity increases with increasing (K+)O from 5 mM to 60 mM and then levels off at higher concentrations. When the (Na+)O concentration was changed from 0 to 137 mM, keeping (K+)O constant at 5 mM, no change is observed in fluorescence. On addition of ethanol, the fluorescence intensity increased. There was a significant change (15%) in fluorescence intensity with as low 10 mM ethanol. The fluorescence intensity increase as ethanol concentration was varied from 10 to 100 mM. Ethanol had no effect of depolarized (80 mM KC1) synaptosomes. The effect of ethanol on membrane depolarization was similar to K+ depolarization. In the absence of synaptosomes ethanol had no effect in the fluorescence intensity of dye. In lysed or 12 hr. old synaptosomes, there was no change in fluorescence either with (K+)O or ethanol. We concluded that (1) Rhodamine 6G fluorescence can be used to monitor the change in membrane potential in synaptosomes (2) low doses of ethanol produces changes in membrane potential in synaptosomes isolated from rat brain.

众所周知，乙醇可以改变生物化学和生物物理