Overcoming mechanical barriers to neuronal regeneration

克服神经元再生的机械障碍

• 批准号: G1100312/1
• 负责人: Kristian Franze
• 金额: $ 142.53万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G1100312%2F1
• 关键词: Overcoming; mechanical; barriers; neuronal; regeneration

After spinal cord injury, many people are wheelchair-bound for the rest of their life. The reason for this dilemma is that neurons, which are crucial cells in the spinal cord connecting the brain with the body, cannot self-repair, and repair can currently not be promoted. So far, research has - without any major breakthrough - mainly focused on chemical signals impeding neuronal re-growth. The proposed project will take an alternative approach: I want to unravel if mechanical barriers are in the way of neuronal repair and find a way how to negotiate these barriers. I will develop a microscopy technique that, for the first time, allows to measure and compare the stiffness of healthy spinal cord tissue with that of scar tissue developing at the injury site. Concurrently, I will investigate the response of nervous system cells to mechanical stimuli and illuminate how these stimuli are translated into a cellular response. Equipped with this knowledge, I will suppress the response of cells in the scar environment to mechanical stimuli. In this manner, I will overcome the negative effect of the scar stiffness and promote neuronal re-growth, which could ultimately lead to the repair of damaged spinal cord tissue.

脊髓损伤后，许多人的余生都必须坐在轮椅上。造成这种困境的原因是，神经元是脊髓中连接大脑与身体的关键细胞，无法自我修复，而且目前无法促进修复。到目前为止，研究主要集中在阻碍神经元再生的化学信号上，没有任何重大突破。拟议的项目将采取另一种方法：我想弄清楚机械障碍是否妨碍神经元修复，并找到一种方法来克服这些障碍。我将开发一种显微镜技术，首次可以测量和比较健康脊髓组织的硬度与损伤部位形成的疤痕组织的硬度。同时，我将研究神经系统细胞对机械刺激的反应，并阐明这些刺激如何转化为细胞反应。有了这些知识，我将抑制疤痕环境中的细胞对机械刺激的反应。通过这种方式，我将克服疤痕僵硬的负面影响并促进神经元重新生长，这最终可能导致受损脊髓组织的修复。