IMMUNOGLOBULIN STRUCTURE & DIVERSITY--CHARACTERIZATION OF CELL MEMBRANE PROTEINS

一、基因进化。重组DNA文库已从一株 各种野生老鼠物种。这些库已经过筛选 与T细胞受体的Beta链相对应的探针 免疫球蛋白kappa轻链恒定区，kappa可变区 基因家族和重链可变区基因家族。数列 近亲关系最远的帕哈里小鼠的这些基因分析 物种到近亲交配的动物，揭示了不同的进化模式和 所研究的每个基因都有突变。因此，选择性和进化性 压力对免疫学的作用似乎完全不同。 重要的结构基因。 二、增长与监管。一种由P388D1类巨噬细胞产生的因子 已鉴定出许多浆细胞瘤所需的细胞系 用于体外生长的细胞系。这一因素目前正在提纯。 并对其进行了表征和实验，以评估其在 浆细胞瘤发育或正常生长。

I. Gene evolution. Recombinant DNA libraries have been constructed from a variety of wild mouse species. These libraries have been screended with probes corresponding to the Beta chain of the T cell receptor, the immunoglobulin kappa light chain constant region, a kappa variable region gene family and a heavy chain variable region gene family. Sequence analysis of these genes from Mus pahari, the most distantly related mouse species to inbred animals, reveals different patterns of evolution and mutation for each of the genes studied. Thus, selective and evolutionary pressures appear to be acting quite differently on immunologically important structural genes. II. Growth and regulation. A factor produced by the P388D1 macrophage-like cell line has been identified which is required by a number of plasmacytoma cell lines for growth in vitro. This factor is currently being purified and characterized and experiments are being initiated to assess its role in plasmacytomagenesis or normal growth.