LYMPHOCYTE CIRCULATION--PLASMACYTOMAGENESIS

BALB/c小鼠对浆细胞瘤的诱导高度敏感(S) 方案，而DBA/2小鼠具有抵抗力(R)。在此之前，我们研究了 BALB/c或DBA/2骨转移的细胞学基础 给SCID小鼠注射骨髓(BM)，并向嵌合体注射Pristane， 或含有未调控的myc和raf癌基因的逆转录病毒J3V1。vbl.使用 这些诱导方案我们确定DBA抗性不能 被敏感的SCID微环境克服提示R/S，in 这个系统，是由捐赠者骨髓决定的。SCID小鼠的研究近况 与外周成熟的BALB/c淋巴细胞单独重组，或 与DBA/2 BM联合转移建议如下：1.)成熟BALB/c B 来自脾、淋巴结或Peyer‘s斑块的细胞同样容易感染 诱导肿瘤；2)DBA/2细胞不抑制BALB/c PCT的发展； 和3.)BALB/c淋巴细胞不能向DBA/2B细胞供应外周血淋巴细胞。 PCT发育所需的细胞信号。因此，DBA/2抗性和 BALB/c对PCT诱导的敏感性是Pct的固有特性 各自的B细胞。二、SCID小鼠也被用来研究正常 淋巴细胞发育和寿命。此前，我们确定 将Peyer‘s斑块细胞过继转移到SCID小鼠体内，结果正常 除胸腺外的所有淋巴组织的重建 仅出现单一阳性的CD_4和CD_8成熟T细胞。我们已经扩大了 这些研究旨在确定这次重组的持续时间和 外周(PLN)和肠系膜(MLN)淋巴的重建潜能 节点单元格。结果表明：1)PLN细胞重建外周 淋巴器官，但在肠道粘膜的再繁殖方面效果较差 组织比Peyer‘s斑块细胞少；PLN和MLN的百里香 重组SCID小鼠含有供体CD4和CD8单一阳性成熟T细胞 细胞；以及4.)PP、MLN和PLN再种群持续时间超过6 月份。这些结果表明，成熟淋巴细胞的寿命 比之前建议的要长。此外，该算法的寻的特性 SCID小鼠体内的这些细胞表明粘膜和外周淋巴细胞 它们调节归巢受体表达的能力不同 因此，他们的淋巴组织重建模式。

BALB/c mice are highly susceptible (S) to plasmacytoma (PCT) induction protocols while DBA/2 mice are resistant (R). Previously we examined the cellular basis for this phenomenon by transferring BALB/c or DBA/2 bone marrow (BM) to SCID mice and injecting the chimeras with either pristane, or the retrovirus J3V1 containing deregulated myc and raf oncogenes. Using these induction protocols we determined that DBA resistance could not be overcome by the susceptible SCID microenvironment suggesting that R/S, in this system, is dictated by the donor BM. Recent studies of SCID mice reconstituted with peripheral, mature BALB/c lymphocytes either alone, or co-transferred with DBA/2 BM suggest the following: 1.) Mature BALB/c B cells from spleen, lymph node or Peyer's patches are equally susceptible to tumor induction; 2.) DBA/2 cells do not suppress BALB/c PCT development; and 3.) BALB/c lymphocytes can not supply DBA/2 B cells with the extra- cellular signals required for PCT development. Thus, DBA/2 resistance and BALB/c susceptibility to PCT induction are traits inherent to the respective B cells. II. SCID mice have also been used to study normal lymphocyte development and life-span. Previously, we determined that adoptive transfer of Peyer's patch cells to SCID mice resulted in normal reconstitution of all lymphoid tissues except the thymus in which there appeared only single positive CD4 and CD8 mature T cells. We have expanded these studies to determine the duration of this reconstitution and the reconstituting potential of peripheral (PLN) and mesenteric (MLN) lymph node cells. Results indicate that: 1.) PLN cells reconstitute peripheral lymphoid organs, but are less effective at repopulation of the gut mucosal tissues than Peyer's patch cells; 3.) The thymii of PLN and MLN reconstituted SCID mice contain donor CD4 and CD8 single positive mature T cells; and 4.) PP, MLN and PLN repopulation persists for greater than 6 months. These results demonstrate that the lifespan of mature lymphocytes is longer than previously suggested. Moreover, the homing properties of these cells in SCID mice indicate that mucosal and peripheral lymphocytes differ in their ability to modulate homing receptor expression and consequently their pattern of lymphoid tissue reconstitution.