LYMPHOCYTE CIRCULATION--PLASMACYTOMAGENESIS

BALB/c小鼠对浆细胞瘤（PCT）诱导高度敏感（S） 方案，而DBA/2小鼠具有抗性（R）。 之前我们检查了 通过转移BALB/c或DBA/2骨， 骨髓（BM）注射到SCID小鼠中，并将植烷， 或含有失调的myc和raf癌基因的逆转录病毒J3V1。 使用 这些诱导方案，我们确定DBA抗性不能被 克服了敏感的SCID微环境，这表明R/S，在 该系统由施主BM规定。 SCID小鼠的最新研究 单独用外周成熟BALB/c淋巴细胞重建，或 与DBA/2 BM共同转让的建议如下：1.）成熟BALB/c B 来自脾、淋巴结或派伊尔集合淋巴结的细胞同样易受 肿瘤诱导; 2.）DBA/2细胞不抑制BALB/c小鼠PCT的发育; 和3.）BALB/c淋巴细胞不能为DBA/2 B细胞提供外源性的 PCT发展所需的细胞信号。 因此，DBA/2抗性和 BALB/c对PCT诱导的易感性是BALB/c小鼠固有的特征， 各自的B细胞。 二. SCID小鼠也被用于研究正常的 淋巴细胞发育和寿命。 此前，我们确定， Peyer’s patch细胞过继转移给SCID小鼠， 所有淋巴组织的重建，除了胸腺， 仅出现单一阳性的CD4和CD8成熟T细胞。 我们扩大 这些研究旨在确定该复溶的持续时间， 外周（PLN）和肠系膜（MLN）淋巴的重建潜力 结细胞 结果表明：1.）PLN细胞重建外周 淋巴器官，但在肠道粘膜的再增殖方面效果较差 组织比Peyer's patch细胞; 3.）PLN和MLN的胸腺 重建的SCID小鼠含有供体CD4和CD8单阳性成熟T细胞 细胞;和4.）PP、MLN和PLN的重新填充持续超过6年 个月 这些结果表明，成熟淋巴细胞的寿命 比以前建议的要长。 此外， 这些细胞表明，粘膜和外周淋巴细胞 它们调节归巢受体表达的能力不同， 因此它们的淋巴组织重建模式。

BALB/c mice are highly susceptible (S) to plasmacytoma (PCT) induction protocols while DBA/2 mice are resistant (R). Previously we examined the cellular basis for this phenomenon by transferring BALB/c or DBA/2 bone marrow (BM) to SCID mice and injecting the chimeras with either pristane, or the retrovirus J3V1 containing deregulated myc and raf oncogenes. Using these induction protocols we determined that DBA resistance could not be overcome by the susceptible SCID microenvironment suggesting that R/S, in this system, is dictated by the donor BM. Recent studies of SCID mice reconstituted with peripheral, mature BALB/c lymphocytes either alone, or co-transferred with DBA/2 BM suggest the following: 1.) Mature BALB/c B cells from spleen, lymph node or Peyer's patches are equally susceptible to tumor induction; 2.) DBA/2 cells do not suppress BALB/c PCT development; and 3.) BALB/c lymphocytes can not supply DBA/2 B cells with the extra- cellular signals required for PCT development. Thus, DBA/2 resistance and BALB/c susceptibility to PCT induction are traits inherent to the respective B cells. II. SCID mice have also been used to study normal lymphocyte development and life-span. Previously, we determined that adoptive transfer of Peyer's patch cells to SCID mice resulted in normal reconstitution of all lymphoid tissues except the thymus in which there appeared only single positive CD4 and CD8 mature T cells. We have expanded these studies to determine the duration of this reconstitution and the reconstituting potential of peripheral (PLN) and mesenteric (MLN) lymph node cells. Results indicate that: 1.) PLN cells reconstitute peripheral lymphoid organs, but are less effective at repopulation of the gut mucosal tissues than Peyer's patch cells; 3.) The thymii of PLN and MLN reconstituted SCID mice contain donor CD4 and CD8 single positive mature T cells; and 4.) PP, MLN and PLN repopulation persists for greater than 6 months. These results demonstrate that the lifespan of mature lymphocytes is longer than previously suggested. Moreover, the homing properties of these cells in SCID mice indicate that mucosal and peripheral lymphocytes differ in their ability to modulate homing receptor expression and consequently their pattern of lymphoid tissue reconstitution.