IMMUNOGLOBULIN STRUCTURE & DIVERSITY--CHARACTERIZATION OF CELL MEMBRANE PROTEINS

1)进行了一系列的实验研究 小鼠白细胞介素6的生物学效应。初步研究 已经证明这一因素可能与B-和B- T细胞发育。IL-6促进胸腺细胞增殖 用凝集素和IL-1治疗。观察到类似的增强。 与IL-4的结合，这些因素的组合已经开始定义 三种特征明确的细胞因子的作用等级。 IL-6在B细胞发育中的作用已经在 对感染血凝素的免疫反应。IL-6抗血清 完全抑制对该抗原的初级反应和 加入纯化的IL-6可逆转抑制作用。在……里面 相反，抗血清对二次反应没有影响 表示在早期对这一因素有强制性要求 B细胞反应的阶段和不同的反应性 初级细胞与次级细胞。 2)已经开始了识别基因和蛋白质的研究 与细胞分化有关。克隆细胞系可以 被诱导分化正在被用来构造减法 用于基因分离的cdna文库。核基质制剂是 同时进行分析以确定 不同发育阶段间蛋白质表达的差异 并有可能分离出特定的蛋白质。

1) A series of experiments have been performed to investigate the biological effects of murine interleukin-6 (IL-6). Initial studies have demonstrated that this factor may be involved in both B- and T-cell development. IL-6 enhances proliferation of thymocytes treated with lectin and IL-1. A similar enhancement is observed with IL-4, and combinations of these factors have begun to define a hierarchy of action for three well-characterized cytokines. The role of IL-6 in B-cell development has been examined in the immune response to influenze hemmagglutinin. Antisera to IL-6 completely inhibit the primary response to this antigen and the inhibition is reversed by the addition of purified IL-6. In contrast, the antisera has no effect on the secondary response indicating an obligatory requirement for this factor in the early stages of the B-cell response and a differential responsiveness of primary versus secondary cells. 2) Studies have been initiated to identify genes and proteins associated with cell differentiation. Clonal cell lines which can be induced to differentiate are being used to construct subtractive cDNA libraries for gene isolation. Nuclear matrix preparations are simultaneously being analyzed to determine the magnitude of expressed protein differences between successive developmental - stages and to potentially isolate specific proteins.