IMMUNOGLOBULIN STRUCTURE & DIVERSITY--CHARACTERIZATION OF CELL MEMBRANE PROTEINS

1)已经进行了一系列实验来研究 小鼠白细胞介素-6（IL-6）的生物学效应。 初步研究 已经证明该因子可能参与B-和 T细胞发育 IL-6促进胸腺细胞增殖 用凝集素和IL-1处理。 观察到类似的增强 与IL-4，这些因素的组合已经开始定义 三种充分表征的细胞因子的作用层次。 IL-6在B细胞发育中的作用已经在 对流感嗜血杆菌免疫应答。 IL-6抗血清 完全抑制对该抗原的初级应答， 通过加入纯化的IL-6来逆转抑制。 在 相反，抗血清对次级反应没有影响 表明在早期对这一因素的强制要求 B细胞反应的不同阶段和 初级细胞与次级细胞。 2)研究已经开始确定基因和蛋白质 与细胞分化有关。 克隆细胞系， 被诱导分化被用来构建消减的 用于基因分离的cDNA文库。 核基质制备是 同时被分析以确定 表达的蛋白质差异， 阶段，并可能分离特定的蛋白质。

1) A series of experiments have been performed to investigate the biological effects of murine interleukin-6 (IL-6). Initial studies have demonstrated that this factor may be involved in both B- and T-cell development. IL-6 enhances proliferation of thymocytes treated with lectin and IL-1. A similar enhancement is observed with IL-4, and combinations of these factors have begun to define a hierarchy of action for three well-characterized cytokines. The role of IL-6 in B-cell development has been examined in the immune response to influenze hemmagglutinin. Antisera to IL-6 completely inhibit the primary response to this antigen and the inhibition is reversed by the addition of purified IL-6. In contrast, the antisera has no effect on the secondary response indicating an obligatory requirement for this factor in the early stages of the B-cell response and a differential responsiveness of primary versus secondary cells. 2) Studies have been initiated to identify genes and proteins associated with cell differentiation. Clonal cell lines which can be induced to differentiate are being used to construct subtractive cDNA libraries for gene isolation. Nuclear matrix preparations are simultaneously being analyzed to determine the magnitude of expressed protein differences between successive developmental - stages and to potentially isolate specific proteins.