The impact of structural dynamics on three-dimensional bioimaging with X-ray free-electron lasers

结构动力学对 X 射线自由电子激光三维生物成像的影响

• 批准号: DE140100624
• 负责人: A/Prof Andrew Martin
• 金额: $ 26.04万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-02-01 至 2017-06-23
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE140100624
• 关键词: impact; structural; dynamics; three; dimensional

X-ray lasers can potentially determine the structures of biological molecules that are inaccessible to existing techniques. Intense ultrafast pulses encode the structure via diffraction faster than damage processes rip the molecule apart. In fact, damage processes begin during diffraction and remain problematic. It is not known if damage will prevent the determination of molecular orientations, a critical step in the experimental design. This project will solve this problem with a statistical theory, probing the feasibility and accuracy of the technique. The newly developed theory will enable us to perform experiments capable of measuring the effects of damage in biological molecules, paving the way for new methods of structure determination.

X射线激光可以确定现有技术无法实现的生物分子的结构。强烈的超快脉冲通过衍射对结构进行编码，速度快于破坏过程将分子撕裂。事实上，损伤过程开始在衍射和仍然是有问题的。目前尚不清楚损伤是否会阻止分子取向的确定，这是实验设计中的关键步骤。该项目将用统计理论解决这个问题，探讨该技术的可行性和准确性。新开发的理论将使我们能够进行能够测量生物分子损伤影响的实验，为结构测定的新方法铺平道路。