From genes to symbionts: arbovirus-vector interactions

从基因到共生体：虫媒病毒-载体相互作用

• 批准号: MC_UU_00034/4
• 负责人: Alain Kohl
• 金额: $ 52.24万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Intramural
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=MC_UU_00034%2F4
• 关键词: genes; symbionts; arbovirus; vector; interactions

Many infectious diseases are transmitted by vectors such as mosquitos, including a set of viruses called arboviruses. Factors such as climate change are increasing the risk of these viruses being transmitted across the globe. Our programme aims to understand the mechanisms of this transmission in three types of arboviruses –Rift Valley fever phlebovirus, the human pathogenic alphavirus chikungunya and dengue virus. These arboviruses have their own ribonucleic acid, or RNA, which is genetic material. Arbovirus RNA binds to proteins in the mosquito ‘host’ and uses them help the virus multiply and cause infection. This infection is then passed on to humans or animals through the mosquito bite. These proteins are known to play a part in human and animal defences against viral infection. However, we don’t know very much these proteins in mosquitos, or how arbovirus RNA may bind to these proteins and stops them from doing their antiviral job. We think that if we can understand these mechanisms in mosquitos, we could find new ways of blocking virus transmission and infection by targeting those binding mechanisms with specific interventions, thus also preventing mosquitos from transmitting the viral infection to humans and animals through their bites.

许多传染病都是通过媒介传播的，如蚊子，包括一组称为虫媒病毒的病毒。气候变化等因素正在增加这些病毒在地球仪传播的风险。我们的计划旨在了解三种虫媒病毒的传播机制-裂谷热静脉病毒，人类致病性甲病毒基孔肯雅和登革热病毒。这些虫媒病毒有自己的核糖核酸，或RNA，这是遗传物质。虫媒病毒RNA与蚊子“宿主”中的蛋白质结合，并利用它们帮助病毒繁殖并引起感染。这种感染通过蚊子叮咬传给人类或动物。已知这些蛋白质在人类和动物抵抗病毒感染的防御中起作用。然而，我们对蚊子中的这些蛋白质了解不多，也不知道虫媒病毒RNA如何与这些蛋白质结合并阻止它们发挥抗病毒作用。我们认为，如果我们能够了解蚊子的这些机制，我们就可以通过针对这些结合机制的特定干预措施来找到阻断病毒传播和感染的新方法，从而防止蚊子通过叮咬将病毒感染传播给人类和动物。

项目成果

Infravec2 guidelines for the design and operation of containment level 2 and 3 insectaries in Europe.

• DOI: 10.1080/20477724.2022.2108639
• 发表时间: 2023-05
• 期刊: PATHOGENS AND GLOBAL HEALTH
• 影响因子: 3.4
• 作者: Pondeville, Emilie;Failloux, Anna-Bella;Simard, Frederic;Volf, Petr;Crisanti, Andrea;Haghighat-Khah, Roya Elaine;Busquets, Nuria;Abad, Francesc Xavier;Wilson, Anthony J.;Bellini, Romeo;Arnaud, Sarah Marsh;Kohl, Alain;Veronesi, Eva
• 通讯作者: Veronesi, Eva

Differences in proteome perturbations caused by the Wolbachia strain wAu suggest multiple mechanisms of Wolbachia-mediated antiviral activity.

• DOI: 10.1038/s41598-023-38127-4
• 发表时间: 2023-07-20
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Rainey, Stephanie M.;Geoghegan, Vincent;Lefteri, Daniella A.;Ant, Thomas H.;Martinez, Julien;McNamara, Cameron J.;Kamel, Wael;de Laurent, Zaydah Rolande;Castello, Alfredo;Sinkins, Steven P.
• 通讯作者: Sinkins, Steven P.

Transgenic expression of cif genes from Wolbachia strain wAlbB recapitulates cytoplasmic incompatibility in Aedes aegypti.

• DOI: 10.1038/s41467-024-45238-7
• 发表时间: 2024-01-29
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Mcnamara, Cameron J.;Ant, Thomas H.;Harvey-Samuel, Tim;White-Cooper, Helen;Martinez, Julien;Alphey, Luke;Sinkins, Steven P.
• 通讯作者: Sinkins, Steven P.

Rift Valley Fever Virus Primes Immune Responses in Aedes aegypti Cells.

• DOI: 10.3390/pathogens12040563
• 发表时间: 2023-04-06
• 期刊: Pathogens (Basel, Switzerland)
• 作者: Laureti M;Lee RX;Bennett A;Wilson LA;Sy VE;Kohl A;Dietrich I
• 通讯作者: Dietrich I

Introduction of Aedes aegypti mosquitoes carrying wAlbB Wolbachia sharply decreases dengue incidence in disease hotspots.

携带 wAlbB 沃尔巴克氏体的埃及伊蚊的引入大大降低了疾病热点地区的登革热发病率。

• DOI: 10.1016/j.isci.2024.108942
• 发表时间: 2024
• 期刊: iScience
• 影响因子: 5.8
• 作者: Hoffmann AA