Deciphering mycolic acid transport in Mycobacterium tuberculosis

破译结核分枝杆菌中分枝菌酸的转运

• 批准号: MR/K00042X/1
• 负责人: Apoorva Bhatt
• 金额: $ 46.23万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FK00042X%2F1
• 关键词: Deciphering; mycolic; acid; transport; Mycobacterium

Tuberculosis is a global health problem, further compounded due to the rise of multi-drug resistance. The causative bacterium, Mycobacterium tuberculosis has a waxy, protective cell wall which is rich in lipids. One such 'building block' of the cell wall is a group of fatty acids termed mycolic acids. While we know how mycolic acids are made, not much is known about how they are transported to the outside of the cell. This study aims to decipher mycolic acid transport processes by using an array of molecular tools that will identify genes responsible for mycolic acid transport. As mycolic acids are essential for the survival of M. tuberculosis, genes identified in this study have potential to be targeted for drug development. Additionally, as mycolic acids are also required for virulence, these studies will, indirectly, also have implications for our understanding of how M. tuberculosis causes disease.

结核病是一个全球性的健康问题，由于多重耐药性的增加而进一步加剧。结核分枝杆菌的致病菌有一层蜡质的保护性细胞壁，富含脂质。细胞壁的一个这样的“构建块”是一组被称为分枝菌酸的脂肪酸。虽然我们知道分枝菌酸是如何产生的，但对它们如何被运输到细胞外知之甚少。本研究的目的是通过使用一系列分子工具来识别负责分枝菌酸转运的基因，从而破译分枝菌酸转运过程。由于分枝菌酸对M.结核病，在这项研究中确定的基因有可能成为药物开发的目标。此外，由于分枝菌酸也是毒力所必需的，这些研究也将间接地对我们理解M。肺结核引起疾病。

项目成果

The mycolic acid reductase Rv2509 has distinct structural motifs and is essential for growth in slow-growing mycobacteria.

分枝菌酸还原酶 Rv2509 具有独特的结构基序，对于缓慢生长的分枝杆菌的生长至关重要。

• DOI: 10.1111/mmi.14437
• 发表时间: 2020
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Javid A

Structure-function analysis of MmpL7-mediated lipid transport in mycobacteria.

• DOI: 10.1016/j.tcsw.2021.100062
• 发表时间: 2021-12
• 期刊: Cell surface (Amsterdam, Netherlands)
• 作者: Moolla N;Bailo R;Marshall R;Bavro VN;Bhatt A
• 通讯作者: Bhatt A

Identification of a Desaturase Involved in Mycolic Acid Biosynthesis in Mycobacterium smegmatis.

• DOI: 10.1371/journal.pone.0164253
• 发表时间: 2016
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Singh A;Varela C;Bhatt K;Veerapen N;Lee OY;Wu HH;Besra GS;Minnikin DE;Fujiwara N;Teramoto K;Bhatt A
• 通讯作者: Bhatt A

Ancient mycobacterial lipids: Key reference biomarkers in charting the evolution of tuberculosis.

古代分枝杆菌脂质：绘制结核病进化图的关键参考生物标志物。

• DOI: 10.1016/j.tube.2015.02.009
• 发表时间: 2015
• 期刊: Tuberculosis (Edinburgh, Scotland)
• 作者: Minnikin DE

The mycobacterial desaturase DesA2 is associated with mycolic acid biosynthesis.

• DOI: 10.1038/s41598-022-10589-y
• 发表时间: 2022-04-28
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Bailo, Rebeca;Radhakrishnan, Anjana;Singh, Albel;Nakaya, Makoto;Fujiwara, Nagatoshi;Bhatt, Apoorva
• 通讯作者: Bhatt, Apoorva