Establishing The Edinburgh Super-Resolution Imaging Consortium (ESRIC)

建立爱丁堡超分辨率成像联盟 (ESRIC)

• 批准号: MR/K01563X/1
• 负责人: Rory Duncan
• 金额: $ 236.34万
• 依托单位: Heriot-Watt University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FK01563X%2F1
• 关键词: Establishing; Edinburgh; Super; Resolution; Imaging

Recently, the detail with which biologists can examine microscopic samples has increased dramatically. Improvements in this microscopic 'resolution' can be split into; temporal (the speed with which biologists can look at fast changing things) and spatial (the size of objects, or spacing between them). Recent advancements in technology, combined with some clever biology, means that we can, for the first time, determine the precise positions of many 10000s of single molecules, their interactions, movements and formation into structures within living cells, with millisecond (1000s of frames of a 'movie' per second) rates. As molecules are the fundamental units of cells, and cells are the minimal units of life, increases in resolution are starting to revolutionise our understanding of cell biology by examining directly the molecular workings of cells.The technological improvements that make this possible have come from the physical sciences: mathematics, physics, chemistry as well as engineering. To continue with this progress, the UK scientific community needs to bring the best cell biologists with the most appropriate biological tools and questions, together with researchers at the cutting edge of the physical sciences. Work at the so-called 'Life Science Interface' is not straightforward; it requires extra time for the various researchers involved to speak to each other, specialised infrastructure, a genuine desire to work across traditional scientific boundaries, as well as committed support from both the funders and the institutional management to ensure that these ambitious collaborations can work. To date, few institutions in the UK can claim success in this.Heriot Watt University is well known internationally for its work in the physical sciences particularly. Taking advantage of this, the University established the 'Life Science Interface Laboratory (LSI)' in 2010 with a large investment in both equipment and staff recruitment. Recruiting biologists with a proven track record of working at the interfaces with mathematics, physics and chemistry, a new Research Institute was established, led by cell biologists, to accelerate the interactions between disciplines. This Institute established a close collaboration with the nearby Institute of Genetics and Molecular Medicine (IGMM), known for its advanced biology aimed at addressing the molecular mechanisms that underly human disorders and diseases. This collaboration is proving very productive, with many small-scale pilot projects under-way investigating the use of the new 'super-resolution' imaging techniques described above, applied to cutting-edge biological questions in human healthcare.Here, we are requesting support for new equipment and technical support to bolster this collaboration. Three new microscopes are requested, which will allow more cell biologists to 'see' structures and molecules in their samples in unprecedented detail. Combined with the facilities at the LSI at Heriot Watt, the 'Edinburgh Super-Resolution Imaging Consortium' (ESRIC) will bring together every single currently available live cell super-resolution imaging approach, together with world-leading cell biologists and physical scientists and engineers with a proven track record of cross-disciplinary successes; something unique in the UK. We are also requesting funding to pursue research across ESRIC, which will in addition be made openly available to a large number of cell biologists across Edinburgh. Importantly, simply purchasing new commercially available microscopes is not the limit of our ambition; the engineers and physicists in our consortium, directed and informed by cell biologists, are already developing the 'next generation' of imaging approaches that will be necessary to continue progress in biological discovery: of equal importance, the senior management of all the Institutes in ESRIC are demonstrably committed to this activity.

最近，生物学家可以检查微观样品的细节已经显着增加。这种微观“分辨率”的提高可以分为：时间（生物学家观察快速变化事物的速度）和空间（物体的大小或它们之间的间距）。最近的技术进步，加上一些聪明的生物学，意味着我们可以，第一次，确定许多10000个单分子的精确位置，它们的相互作用，运动和形成活细胞内的结构，毫秒（每秒1000帧的“电影”）速率。由于分子是细胞的基本单位，而细胞是生命的最小单位，分辨率的提高开始通过直接检查细胞的分子工作来彻底改变我们对细胞生物学的理解。使这成为可能的技术进步来自物理科学：数学，物理，化学以及工程。为了继续这一进展，英国科学界需要将最好的细胞生物学家与最合适的生物学工具和问题，以及物理科学前沿的研究人员联系起来。在所谓的“生命科学接口”的工作并不简单;它需要额外的时间让参与的各种研究人员相互交谈，专业的基础设施，跨越传统科学界限的真正愿望，以及来自资助者和机构管理层的承诺支持，以确保这些雄心勃勃的合作能够发挥作用。迄今为止，英国很少有机构能在这方面取得成功。埃里奥特瓦特大学以其在物理科学方面的工作而闻名于世。利用这一优势，大学于2010年建立了“生命科学接口实验室（LSI）”，在设备和人员招聘方面进行了大量投资。招募在数学，物理和化学接口工作的良好记录的生物学家，成立了一个新的研究所，由细胞生物学家领导，以加速学科之间的互动。该研究所与附近的遗传学和分子医学研究所（IGMM）建立了密切的合作关系，该研究所以其先进的生物学而闻名，旨在解决人类疾病和疾病的分子机制。这种合作被证明是非常富有成效的，许多小规模的试点项目正在调查使用上述新的“超分辨率”成像技术，应用于人类医疗保健中的尖端生物学问题。在这里，我们请求支持新设备和技术支持，以加强这种合作。三种新的显微镜被要求，这将使更多的细胞生物学家能够以前所未有的细节“看到”样品中的结构和分子。结合埃里奥特瓦特LSI的设施，“爱丁堡超分辨率成像联盟”（ESRIC）将汇集目前可用的每一种活细胞超分辨率成像方法，以及世界领先的细胞生物学家和物理科学家和工程师，他们拥有跨学科成功的良好记录;这在英国是独一无二的。我们还请求资助在ESRIC进行研究，此外，该研究还将向爱丁堡的大量细胞生物学家公开提供。重要的是，仅仅购买新的商用显微镜并不是我们雄心的极限;我们联盟中的工程师和物理学家，在细胞生物学家的指导和告知下，已经在开发“下一代”成像方法，这对于继续生物发现的进展是必要的：同样重要的是，ESRIC所有研究所的高级管理层都明确致力于这一活动。

项目成果

SBE6: a novel long-range enhancer involved in driving sonic hedgehog expression in neural progenitor cells.

• DOI: 10.1098/rsob.160197
• 发表时间: 2016-11
• 期刊: Open biology
• 影响因子: 5.8
• 作者: Benabdallah NS;Gautier P;Hekimoglu-Balkan B;Lettice LA;Bhatia S;Bickmore WA
• 通讯作者: Bickmore WA

Navigation through the Plasma Membrane Molecular Landscape Shapes Random Organelle Movement.

• DOI: 10.1016/j.cub.2016.12.002
• 发表时间: 2017-02-06
• 期刊: Current biology : CB
• 作者: Dun AR;Lord GJ;Wilson RS;Kavanagh DM;Cialowicz KI;Sugita S;Park S;Yang L;Smyth AM;Papadopulos A;Rickman C;Duncan RR
• 通讯作者: Duncan RR

A negative loop within the nuclear pore complex controls global chromatin organization.

• DOI: 10.1101/gad.264341.115
• 发表时间: 2015-09-01
• 期刊: Genes & development
• 影响因子: 10.5
• 作者: Breuer M;Ohkura H
• 通讯作者: Ohkura H

PSD95 nanoclusters are postsynaptic building blocks in hippocampus circuits.

• DOI: 10.1038/srep24626
• 发表时间: 2016-04-25
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Broadhead MJ;Horrocks MH;Zhu F;Muresan L;Benavides-Piccione R;DeFelipe J;Fricker D;Kopanitsa MV;Duncan RR;Klenerman D;Komiyama NH;Lee SF;Grant SG
• 通讯作者: Grant SG

SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy.

• DOI: 10.1038/srep15915
• 发表时间: 2015-11-03
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Ball G;Demmerle J;Kaufmann R;Davis I;Dobbie IM;Schermelleh L
• 通讯作者: Schermelleh L