CORE--CELLULAR RESOURCES

该核心将执行细胞分离和培养程序， 项目2、3和4的研究人员。 核心将负责 心肌细胞的分离和维持， 非洲爪蟾卵母细胞，并进行细胞质注射 由分子生物学核心提供的mRNA或DNA的核注射 (Core B）。 项目2和项目3都提出了对人类和大鼠钾的研究 在小鼠Ltk细胞中表达的通道;本核心将假设 培养和维护这些文化的责任 在核心B中进行了转染。 通过集中这些例行的 实验室职能、资源（研究者努力、动物数量和 成本将得到更有效的利用。

This Core will perform cell isolations and culture procedures for investigators in Projects 2, 3, and 4. The Core will be responsible for isolating and maintaining cardiac myocytes, isolating and maintaining Xenopus laevis oocytes, and carrying out either cytoplasmic injections of mRNA or nuclear injections of DNA provided by the Molecular Biology Core (Core B). Projects 2 and 3 both propose studies of human and rat potassium channels expressed in mouse Ltk cells; this Core will assume the responsibility of growing and maintaining these cultures after the initial transfections have been performed in Core B. By centralizing these routine laboratory functions, resources (investigator effort, animal numbers and costs) will be used more effectively.