MICA: Open-access biomaterials microfabrication and non-invasive imaging facilities for Regenerative Medicine.

MICA：用于再生医学的开放式生物材料微加工和非侵入性成像设施。

• 批准号: MR/L012669/1
• 负责人: Sheila MacNeil
• 金额: $ 93.11万
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FL012669%2F1
• 关键词: MICA; Open; access; biomaterials; microfabrication

We propose a transformative instrumentation bid to catalyse collaborative regenerative medicine research. We are requesting to purchase capital equipment which will allow the regenerative medicine community in Sheffield (who are all members of the University Centre for Biomaterials and Tissue Engineering) and the UK to keep at the forefront of internationally competitive research. Between them the applicants have experience of tackling clinical problems, which are not currently amenable to conventional treatment - MacNeil has developed tissue engineered skin for patients with extensive skin loss due to burns injuries and commercialised this as a project (MySkin). She has also developed tissue engineered buccal mucosa and translated this into early stage clinical evaluation for patients with scarring of the urethra. She is now working with colleagues in India to develop a simpler and safer alternative to the amniotic membrane, which is currently used to culture corneal stem cells for transfer to the clinic for patients with scarring of the cornea. This work on microfabricating a rapidly degrading scaffold (with Claeyssens) has also led to a new methodology for scaling up and speeding up the projection of electrospun scaffolds which contain 3D structures to protect these fragile cells. Haycock has made significant breakthroughs in how to culture stem cells for peripheral nerve repair and working with Claeyssens has developed nerve tissue guides into which these cells are placed. This work is showing significant promise for nerve regeneration in animal models of nerve transection, with plans for clinical translation. The development of these tissue-engineering technologies is very much underpinned by current instrumentation and expertise within the laboratory, but has been strengthened in recent years with the appointment of Matcher who has international expertise in biophotonics, and this is being applied to imaging 3D tissue constructs. The purchase of new equipment will allow this work to progress into the clinic to image cells and constructs post implantation in the cornea, skin and nerve. Claeyssens brings expertise in microfabrication which has already led to significant advances in ongoing programmes of cornea and nerve repair and has the potential to advance to the production of patient specific constructs and to scale up the production of constructs for clinical trials. Rehman brings a wealth of expertise on FTIR and Raman spectroscopy of biological tissues, including bone, cell, tissues and histopathologically fixed biopsies, and these techniques are currently being applied to investigating 3D constructs of skin and analysing surface properties of scaffolds. In addition, his expertise in developing bioactive-guided regenerative membranes for various applications, such as periodontal treatment and restorative materials adds considerably to the biomaterials manufacturing portfolio of this centre. The latter technique is information rich while imaging cells with optical coherence tomography has relatively poor resolution but is fast and convenient and can be translated to the clinic. We anticipate synergy between having both technologies available.Thus, the applicants have between them a range of applications and instrumentation technologies to address problems in regenerative medicine. The equipment requested will not only support these projects but will benefit other members of the UK Regenerative Medicine community both locally and across the UK research community. This will be achieved by running the technologies as open access facilities for the UK research community, as it is unlikely that all such Centres in the UK will have access to microfabrication and state-of the art non-invasive imaging facilities. These facilities underpinned by the research expertise available in Sheffield will lead to new collaborations and will benefit UK Regenerative Medicine.

我们提出了一个变革性的仪器投标，以催化合作再生医学研究。我们正在请求购买资本设备，这将使再生医学界在谢菲尔德（谁是生物材料和组织工程大学中心的所有成员）和英国保持在国际竞争力的研究前沿。他们之间的申请人有解决临床问题的经验，这是目前不适合传统的治疗-麦克尼尔已经开发了组织工程皮肤的患者广泛的皮肤损失，由于烧伤和商业化，这作为一个项目（MySkin）。她还开发了组织工程颊粘膜，并将其转化为尿道瘢痕患者的早期临床评估。她现在正在与印度的同事合作，开发一种更简单、更安全的羊膜替代品，目前羊膜用于培养角膜干细胞，以便转移到角膜瘢痕患者的诊所。这项关于微制造快速降解支架的工作（与Claeyssens一起）也导致了一种新的方法，用于扩大和加速含有3D结构的电纺支架的投影，以保护这些脆弱的细胞。Haycock在如何培养用于外周神经修复的干细胞方面取得了重大突破，并与Claeyssens合作开发了神经组织引导器，将这些细胞放置其中。这项工作显示出在神经横断动物模型中神经再生的重要前景，并计划用于临床转化。这些组织工程技术的发展在很大程度上得到了实验室内现有仪器和专业知识的支持，但近年来随着Matcher的任命而得到加强，Matcher在生物光子学方面具有国际专业知识，这被应用于成像3D组织结构。购买新设备将使这项工作能够进入诊所，对角膜、皮肤和神经植入后的细胞和结构进行成像。Claeyssens带来了微细加工方面的专业知识，这已经导致了正在进行的角膜和神经修复计划的重大进展，并有可能推进到患者特定结构的生产，并扩大临床试验结构的生产。Alberman在生物组织的FTIR和拉曼光谱学方面拥有丰富的专业知识，包括骨、细胞、组织和组织病理学固定的活检组织，这些技术目前正被应用于研究皮肤的3D结构和分析支架的表面特性。此外，他在开发用于各种应用的生物活性引导再生膜方面的专业知识，如牙周治疗和修复材料，大大增加了该中心的生物材料制造组合。后一种技术信息丰富，而光学相干断层扫描成像细胞具有相对较差的分辨率，但快速方便，可以转化为临床。我们期望这两种技术之间的协同作用。因此，申请人之间有一系列的应用和仪器技术，以解决再生医学中的问题。所要求的设备不仅将支持这些项目，而且将使英国再生医学社区的其他成员受益，无论是在当地还是在整个英国研究界。这将通过将这些技术作为英国研究界的开放获取设施来实现，因为英国的所有此类中心都不太可能获得微加工和最先进的非侵入性成像设施。这些设施以谢菲尔德现有的研究专业知识为基础，将导致新的合作，并将使英国再生医学受益。

项目成果

Synergistic effect of type and concentration of surfactant and diluting solvent on the morphology of emulsion templated matrices developed as tissue engineering scaffolds

• DOI: 10.1016/j.reactfunctpolym.2022.105387
• 发表时间: 2022-09-13
• 期刊: REACTIVE & FUNCTIONAL POLYMERS
• 影响因子: 5.1
• 作者: Dikici, Betul Aldemir;Dikici, Serkan;Claeyssens, Frederik
• 通讯作者: Claeyssens, Frederik

Elastomeric, bioadhesive and pH-responsive amphiphilic copolymers based on direct crosslinking of poly(glycerol sebacate)-co-polyethylene glycol.

• DOI: 10.1039/d2bm01335e
• 发表时间: 2022-12-06
• 期刊: BIOMATERIALS SCIENCE
• 影响因子: 6.6
• 作者: Aleemardani, Mina;Trikic, Michael Zivojin;Green, Nicola Helen;Claeyssens, Frederik
• 通讯作者: Claeyssens, Frederik

Synthesis and characterisation of photocurable poly(glycerol sebacate)-co-poly(ethylene glycol) methacrylates

• DOI: 10.1016/j.mtadv.2023.100410
• 发表时间: 2023-08
• 期刊: Materials Today Advances
• 影响因子: 10
• 作者: Mina Aleemardani;Louis Johnson;M. Trikić;N. Green;F. Claeyssens

In Vivo Bone Regeneration Capacity of Multiscale Porous Polycaprolactone-Based High Internal Phase Emulsion (PolyHIPE) Scaffolds in a Rat Calvarial Defect Model.

• DOI: 10.1021/acsami.3c04362
• 发表时间: 2023-06-14
• 期刊: ACS APPLIED MATERIALS & INTERFACES
• 影响因子: 9.5
• 作者: Aldemir Dikici, Betul;Chen, Min-Chia;Dikici, Serkan;Chiu, Hsien-Chung;Claeyssens, Frederik
• 通讯作者: Claeyssens, Frederik

Emulsion templated scaffolds manufactured from photocurable polycaprolactone

• DOI: 10.1016/j.polymer.2019.05.023
• 发表时间: 2019-06-26
• 期刊: POLYMER
• 影响因子: 4.6
• 作者: Dikici, Betul Aldemir;Sherborne, Colin;Claeyssens, Frederik
• 通讯作者: Claeyssens, Frederik