Role of RNA repair in the tolerance of bacteria to antibiotics.

RNA 修复在细菌对抗生素耐受性中的作用。

• 批准号: MR/M017672/1
• 负责人: Martin Buck
• 金额: $ 31.33万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FM017672%2F1
• 关键词: Role; RNA; repair; tolerance; bacteria

The killing of bacteria by antibiotics is preceded by a period of adaptation and tolerance to the antibiotic(s). Similarly sub lethal amounts of antibiotics cause adaptations in the bacterial cells allowing them to better survive in the presence of antibiotics. Identifying the mechanisms behind antibiotic tolerance will afford us with new ways to manage the administration of antibiotics for controlling infections, and also inform new strategies for enhancing the action of antibiotics. We have discovered certain antibiotics cause the induction of a repair system in bacterial cells. The system repairs particular molecules in the cell called RNA, which perform key functions for cell growth and maintenance. By working out how the cells use the RNA repair system to help them survive antibiotic challenges we will be able to plan how to use different specific combinations of antibiotics to kill the bacteria, and also embark on a program of work to consider inhibiting the repair system itself. We anticipate that we can potentiate the killing actions of existing antibiotics through careful establishment of appropriate combinatorial treatments of bacterial infections.

抗生素杀死细菌之前，需要一段时间的适应和耐受。类似地，亚致死量的抗生素会引起细菌细胞的适应，使它们在抗生素存在下更好地生存。确定抗生素耐受性背后的机制将为我们提供新的方法来管理抗生素的管理以控制感染，并为增强抗生素作用的新策略提供信息。我们已经发现某些抗生素可以诱导细菌细胞中的修复系统。该系统修复细胞中称为RNA的特定分子，这些分子执行细胞生长和维护的关键功能。通过研究细胞如何使用RNA修复系统来帮助它们在抗生素的挑战中生存下来，我们将能够计划如何使用不同的抗生素组合来杀死细菌，并开始考虑抑制修复系统本身的工作计划。我们预期，我们可以加强现有抗生素的杀伤作用，通过仔细建立适当的组合治疗细菌感染。

项目成果

Inferring extrinsic noise from single-cell gene expression data using approximate Bayesian computation.

• DOI: 10.1186/s12918-016-0324-x
• 发表时间: 2016-08-22
• 期刊: BMC systems biology
• 作者: Lenive O;W Kirk PD;H Stumpf MP
• 通讯作者: H Stumpf MP

The RNA repair proteins RtcAB regulate transcription activator RtcR via its CRISPR-associated Rossmann fold domain.

• DOI: 10.1016/j.isci.2022.105425
• 发表时间: 2022-11-18
• 期刊: ISCIENCE
• 影响因子: 5.8
• 作者: Kotta-Loizou, Ioly;Giuliano, Maria Grazia;Jovanovic, Milija;Schaefer, Jorrit;Ye, Fuzhou;Zhang, Nan;Irakleidi, Danai Athina;Liu, Xiaojiao;Zhang, Xiaodong;Buck, Martin;Engl, Christoph
• 通讯作者: Engl, Christoph

Cellular and molecular phenotypes depending upon the RNA repair system RtcAB of Escherichia coli.

• DOI: 10.1093/nar/gkw628
• 发表时间: 2016-11-16
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Engl C;Schaefer J;Kotta-Loizou I;Buck M
• 通讯作者: Buck M

In vitro and in vivo methodologies for studying the Sigma 54-dependent transcription.

用于研究 Sigma 54 依赖性转录的体外和体内方法。

• DOI: 10.1007/978-1-4939-2392-2_4
• 发表时间: 2015
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Buck M