Single Cell-Level Functional Proteomics and Genomics exemplified in Cancer and Immunology

我们建议建立一个最先进的设施，以研究与疾病过程有关的单个人类细胞的功能和性质，结合细胞分离和基因和蛋白质分析的最新发展。该系统将与工业伙伴合作开发，并通过在免疫介导疾病、再生医学和癌症方面的联合研究项目来举例说明。这些提供了不同的挑战，但有显著的串扰，使它们成为探索设施全部潜力的理想选择。例如，癌症的生长和扩散的特点是细胞的变异来自“自我”发展逃避免疫系统的方式；而自身免疫性和炎症性疾病（糖尿病、关节炎）的出现是因为免疫系统以自身为目标。此外，在移植和再生医学中，对自体和新自体细胞的反应是移植物接受的主要障碍。通过新技术平台的协同开发，在一个拥有所有相关专业知识和相关基础设施的单一位点上分析癌症、炎症、自身免疫和移植的复杂细胞过程，我们的目标是在单细胞水平上解决复杂的疾病过程。该联盟汇集的专业知识和方法为在重叠的系统中共享技术利益创造了机会。我们的目标是，单细胞水平功能蛋白质组学和基因组学系统一旦开发出来，将加速有关疾病过程、治疗和监测的临床重要发现，并作为在一系列临床领域的卓越中心建立类似设施的典范。

We propose to create a state-of-the-art facility for studying the function and nature of single human cells involved in disease processes, combining the latest developments in cell isolation and analysis of genes and proteins. The system will be developed in collaboration with industrial partners, and exemplified via cohesive research programmes in immune-mediated disease, regenerative medicine and cancer. These provide distinct challenges but have significant cross-talk, making them ideal for exploring the full potential of the facility. For example, cancer growth and spread are characterised by variants of cells from "self" developing ways of evading the immune system; whilst autoimmune and inflammatory disease (diabetes, arthritis) arises because the immune system targets self. Moreover, in transplantation and regenerative medicine, responses to self and neo-self cells are a major barrier to graft acceptance. Through the synergistic development of new technology platforms that analyse complex cellular processes in cancer, inflammation, autoimmunity and transplantation on a single site that houses all of the relevant expertise and related infrastructure already, we aim to resolve complex disease processes at the single cell level. The expertise and methodologies brought together by the consortium create an opportunity to share technological benefits in an overlapping set of systems. Our goal is that the Single Cell-Level Functional Proteomics and Genomics system, once developed, will accelerate clinically important discoveries about disease processes, their treatment and monitoring, and act as a model for the establishment of similar facilities in centres of excellence across a range of clinical arenas.