IONIC MECHANISMS OF SPONTANEOUSLY ACTIVE AMINERGIC NEURO

这个项目的总体目标是：(i)研究产生攻击性状态变化的外周中枢神经系统机制和相互作用（使用美国龙虾作为甲壳类动物模型系统）；（ii）确定影响攻击行为的单个神经分泌神经元的生理和形态学基础。5 -羟色胺神经元，负责调节攻击龙虾已确定在形态和生理水平。具体来说，我将重点关注神经分泌神经元的一个子集，即自发性血清素神经分泌细胞（A1-5HT）。离子电流的性质决定了这些细胞的自发性将被识别和量化。此外，我们还将进一步探讨灌注5 -羟色胺和20-羟基蜕皮激素对含Al神经节分泌的神经分泌神经元离子通道特性的生理影响。标准电生理学结合电压箝位（NRSA资助期间学习的主要技术）将用于描述离子电流彼此之间的关系以及它们与中枢和周围神经系统行为调节相关的相互作用。总之，这些神经分泌细胞的离子通道特性将被检查和鉴定。

The general goal of this project is to (i) examine the peripheral central nervous system mechanisms and interactions which produce changes in aggressive state (using the American lobster, as the crustacean model system); and (ii) to determine the physiological and morphological basis of single neurosecretory neurons affecting aggressive behavior. Serotonin neurons, responsible for modulating aggression in lobsters have been identified at a morphological and physiological level. Specifically, I will focus on a sub-set of neurosecretory neurons, namely the spontaneous serotonin neurosecretory cells (A1-5HT). The properties of the ionic currents which determine spontaneity of these cells will be identified and quantified. Furthermore, the physiological affect of perfused serotonin and 20-hydroxyecdysone, the crustacean molting hormone, on the ionic channel properties of Al ganglion-secretory containing neurosecretory neurons, will be further explored. Both standard electrophysiology combined with voltage clamping (the major technique to be learnt in this NRSA grant period), will be used to describe the relationship of ionic currents with each other and their interactions relating to behavioral modulation at both the central and peripheral nervous systems. In summary, the ionic channel properties of these neurosecretory cells will be examined and identified.