Genetics and neurobiology of aggression of Betta splendens

芨芨草攻击行为的遗传学和神经生物学

• 批准号: 10731186
• 负责人: Pei Yin Shih
• 金额: $ 12.5万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10731186
• 关键词: 3D Print; Affect; Aggressive behavior; Amygdaloid structure; Animal Behavior; Animals; Behavior; Behavioral; Brain region; Breeding; CRISPR/Cas technology; Cessation of life; Chromosome Mapping; Classification; Code; Custom; Data; Development; Development Plans; Economic Burden; Etiology; Excitatory Synapse; Faculty; Fishes; Foundations; Frequencies; Generations; Genes; Genetic; Genetic Variation; Genomics; Gills; Goals; Heritability; Human; Image; Individual; Individual Differences; Infrastructure; Inhibitory Synapse; Knock-out; Knowledge; Lung; Machine Learning; Maintenance; Maps; Mediating; Mentorship; Methods; Mind; Modeling; Molds; Molecular; Mollies; Mus; Nervous System; Neurobiology; Neurons; Partner in relationship; Pathway interactions; Pattern; Performance; Phase; Physiological; Population Genetics; Positioning Attribute; Quantitative Trait Loci; Recording of previous events; Research; Research Activity; Research Personnel; Resource Development; Resources; Robot; Ruta; Shapes; Signal Transduction; Social Behavior; Societies; Specific qualifier value; Statistical Methods; Synapses; System; Techniques; Technology; Testing; Tracer; Transgenic Organisms; Variant; Work; autism spectrum disorder; base; behavior test; behavioral response; brain behavior; career; career development; erection; falls; fighting; gain of function; gene discovery; genetic approach; genetic variant; innovation; insight; interdisciplinary approach; interest; neural; neural circuit; neuroligin 3; neuronal circuitry; neuropsychiatry; novel; novel strategies; overexpression; siamese fighting fish; skills; tool; trait; visual stimulus

Project Summary / Abstract Project description: Aggression is a competitive social behavior that animals use to acquire resources and protect kin. While genetic variation is known to contribute to individual differences in aggression, we know very little about the specific genes involved and their mechanisms. To discover genes that regulate aggression, the applicant will exploit the unique features of the Siamese fighting fish Betta splendens (betta). Highly aggressive fighting betta have been generated through a long history of artificial breeding. Their aggressive behaviors are easily triggered by visual stimuli, and their intense fighting continues until retreat or death. The applicant proposes to use these fighting fish, along with their unselected, less aggressive wild counterparts, to expand our knowledge of genetic and neuronal bases underlying aggression. In Aim 1 (K99), the applicant will characterize the behavioral differences between wild and fighting betta during fights by developing a novel behavioral test and utilizing machine-learning based tools for behavioral analyses. In Aim 2 (K99), the applicant will discover aggression-associated variants that contribute to the behavioral differences with complementary mapping methods and genetic tools for functional perturbation. Finally, in Aim 3 (R00), the applicant will probe how the mapped genes influence the nervous system and mediate aggression using neuronal imaging and circuit tracing tools. Candidate and career goals: The applicant’s broad research interests involve understanding how genetic variation shapes the nervous system to modulate behaviors. The proposed research activities will build upon the applicant’s strong background in genetics and genomics skillsets with imaging and circuit tracing techniques, enabling the interdisciplinary approach necessary to tackle the applicant’s highly innovative research questions. The proposed project will also lay the foundation for the applicant’s long-term goal as an independent researcher who brings computational, genetic, and neurobiological fields together to create bold new approaches for understanding social behavior. Career development plan: The K99 phase of this work will be done in Columbia’s Zuckerman Mind Brain Behavior Institute (ZMBBI) under the mentorship of Drs. Andrés Bendesky and Franck Polleux. In addition, the candidate has assembled an extraordinary team of advisors and collaborators, consisting of Drs. Molly Przeworski, Carolyn (Lindy) McBride, Vanessa Ruta, and David Manuel Flores Gonçalves. The applicant’s research and transition to independence will benefit from this strong mentorship team, the state-of-the-art facilities and infrastructure at ZMBBI, and numerous Professional Development resources offered through ZMBBI and Columbia.

项目总结/摘要 项目描述：攻击是动物用来获取资源和资源的竞争性社会行为， 保护亲属虽然遗传变异是众所周知的，有助于个体差异的侵略，我们知道非常 很少有关于参与的特定基因及其机制的研究。为了发现调节攻击性的基因， 申请人将利用暹罗斗鱼Betta splendens（斗鱼）的独特特征。高度侵袭性 斗鱼是经过长期人工繁殖而产生的。他们的攻击行为 它们很容易被视觉刺激所触发，激烈的战斗一直持续到撤退或死亡。申请人 建议利用这些斗鱼，沿着它们的凶猛，不那么咄咄逼人的野生同类， 我们对遗传和神经元基础的知识是侵略性的基础。在目的1（K99）中，申请人须 描述野生斗鱼和斗鱼在打斗中的行为差异， 行为测试和利用基于机器学习的工具进行行为分析。在目标2（K99）中， 申请人将发现与攻击相关的变体，这些变体有助于行为差异， 互补映射方法和遗传工具的功能扰动。最后，在目标3（R 00）中， 申请人将探索映射的基因如何影响神经系统和介导的侵略性， 神经成像和电路追踪工具。 候选人和职业目标：申请人广泛的研究兴趣涉及了解基因是如何遗传的。 变异塑造神经系统以调节行为。拟议的研究活动将建立在 申请人在遗传学和基因组学技能方面的强大背景，包括成像和电路跟踪 技术，使必要的跨学科的方法来解决申请人的高度创新 研究问题。拟议的项目还将为申请人的长期目标奠定基础 一个独立的研究人员，他把计算、遗传和神经生物学领域结合在一起， 理解社会行为的新方法。 职业发展计划：这项工作的K99阶段将在哥伦比亚的Zuckerman Mind Brain中完成 在Andrés Bendesky和Franck Polleux博士的指导下，参加了行为研究所（ZMBBI）。此外该 候选人已经组建了一个由顾问和合作者组成的非凡团队，其中包括莫莉博士 Przeworski，Carolyn（Lindy）McBride，Vanessa Ruta和大卫Manuel弗洛雷斯Gonçalves。申请人的 研究和过渡到独立将受益于这个强大的导师团队，国家的最先进的 ZMBBI的设施和基础设施，以及通过 ZMBBI和哥伦比亚。