MOLECULAR MECHANISMS OF NEURONAL MORPHOGENESIS

描述了解神经元形态发生的分子基础对于解决各种神经生物学问题非常重要，从功能性神经系统的建立到参与学习和记忆的神经元的结构可塑性。为了研究果蝇中枢神经系统中神经元是如何获得其复杂形态的，已经开发了一种新的遗传镶嵌系统，用于特异性地标记在野生型背景中遗传改变的神经元的形态。使用这个新开发的系统，人们可以密切关注单个野生型或突变神经元的形态发生过程。为了进一步阐明神经元形态发生的分子机制，在开发野生型突变神经元的细胞骨架组织的各个方面将通过在这个新的遗传嵌合体系统中使用不同的标记物，用单细胞分辨率进行检查。此外，蘑菇体的发育，特别是变态过程中神经母细胞增殖和神经元重组的调节，将被调查，这是重要的，为未来的遗传筛选诺埃尔基因参与蘑菇体发育。由于蘑菇体在学习记忆中的重要作用，本研究也将有助于阐明学习记忆的分子机制。

DESCRIPTION Understanding the molecular basis of neuronal morphogenesis is important for addressing various neurobiological questions, from the establishment of a functional nervous system to the structural plasticity of neurons involved in learning and memory. To study how neurons acquire their complicated morphology in the Drosophila central nervous system, a novel genetic mosaic system has been developed for specifically labeling the morphology of genetically altered neurons in an otherwise wild type background. Using this newly developed system, one can closely follow the morphogenetic process of individual wild type or mutant neurons. To elucidate further the molecular mechanisms of neuronal morphogenesis, various aspects of cytoskeleton organization in developing wild type of mutant neurons will be examined with single cell resolution by using different markers in this new genetic mosaic system. Additionally, development of the mushroom body, especially regulation of neuroblast proliferation and reorganization of neurons during metamorphosis, will be investigated, which is important for future genetic screening of noel genes involved in mushroom body development. Cause of the essential role of the mushroom body in learning and memory, this study will also help to elucidate the molecular mechanisms of learning and memory.