MOLECULAR PROBES TO IMAGE DNA DAMAGE IN BRAIN CELLS

提出了一种新的检测和分析方法 组织切片和活体组织中的DNA凋亡性损伤。这项技术是 基于它的DNA探针的原位连接将唯一结合到 特征性的DNA断裂，其他人和我们已经证明是一种特定的 代表细胞凋亡的签名。初步结果使用第一代 与其他方法相比，探针检测细胞凋亡具有更高的特异性 目前使用的基于末端转移酶的标记(TUNEL)，从而证明 开发成用于组织切片的适销对路试剂盒的潜力。 正在开发的探针包括无环发夹寡核苷酸探针，具有更大的 特异性和较少的背景染色，以及利用探针 荧光共振能量转移(FRET)技术，变色 在成功结扎后。技术的一个更强大的应用是 与细胞凋亡的活体成像相关。在初步实验中，我们测试了 对该方法进行了改进，使其能够检测体内特定的DNA损伤。vbl.使用 这种改进的方法，我们将开发一种有市场的体内检测程序，用于 利用磁共振进行可视化，分析大脑中的细胞凋亡损伤 成像(MRI)。 建议的商业应用：不可用

A novel approach is proposed for detection and analysis of apoptotic DNA damage in tissue sections, as well as in vivo. The technology is based on it situ ligation of DNA probes that will uniquely bind to characteristic DNA breaks, which other and we have demonstrated are a specific signature for apoptosis. Preliminary results using the first generation of the probes documented higher specificity for apoptosis detection compared with the currently used terminal transferase-based labeling (TUNEL), thus demonstrating the potential for development into a marketable kit for use in tissue sections. Probes under development include loopless hairpin oligoprobes with greater specificity and less background staining, as well as probes utilizing Fluorescence Resonance Energy Transfer (FRET) technology, which change color upon successful ligation. A much more powerful application of technology is related to in vivo imaging of apoptosis. In preliminary experiments we have tested the modification of the approach allowing it to detect specific DNA damage in vivo. Using this modified approach, we will develop a marketable in vivo detection procedure for analysis of apoptotic damage in brain, with visualization utilizing magnetic resonance imaging (MRI). PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE