OPTIMIZE HUMAN NEURONAL CELLS FOR SPINAL CORD TRANSPLANT

描述：（逐字引用申请人摘要）神经元置换 治疗是治疗脊髓损伤的有希望的方法。的 hNT-神经元的独特性质使TEM成为这种细胞的潜在来源。 目的. hNT-神经元是纯的、有丝分裂后的、分化的人神经元 根据良好的，可以无限量生产的细胞 生产实践。在移植实验中，这些神经元存活下来， 成熟，生长神经突，形成突触，整合，恢复功能。的 这项研究是基于对神经元可塑性的认识 以及它们多样的神经递质表型， 最佳脊髓替代神经元的发育。目标是改变 分化过程的参数，以产生hNT神经元， 选择的表型，筛选特定神经元制剂的活性， 脊髓组织块体外培养，并评估优化的hNT-神经元 在体内脊髓损伤的动物模型中。初步证据表明 hNT神经元在科学上是有前途的，技术上是可行的， 作为替代神经元的来源用于治疗 脊髓损伤和其他CNS疾病。 拟议商业应用：不可用

DESCRIPTION: (Verbatim from the Applicant's Abstract) Neuronal replacement therapy is a promising approach for the treatment of spinal cord injury. The unique properties of hNT-Neurons make tem a potential source of cells for this purpose. hNT-Neurons are pure, post-mitotic, differentiated human neuronal cells that can be produced in unlimited quantities according to Good Manufacturing Practices. In transplantation experiments, these neurons survive, mature, grow neurites, form synapses, integrate, and restore functioning. The proposed research is bas4ed on an appreciation of the plasticity of the neurons and their diverse neurotransmitter phenotypes, important properties for the development of optimal spinal cord replacement neurons. The aims are to vary the parameters of the differentiation process to produce hNT-Neurons with selected phenotypes, screen the activity of specific neuronal preparations in spinal cord explant cultures in vitro, and evaluate the optimized hNT-Neurons in an animal model of spinal cord injury in vivo. Preliminary evidence suggests that hNT-Neurons are scientifically promising, technically feasible, and commercially viable as a source of replacement neurons for the treatment of spinal cord injury and other CNS disorders. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE