CORE--CELL BIOLOGY

核心--细胞生物学

• 批准号: 6110293
• 负责人: PHILIP L LEOPOLD
• 金额: $ 25.26万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6110293
• 关键词: bioimaging /biomedical imaging; biomedical facility; biotechnology; confocal scanning microscopy; cystic fibrosis; cytology; fluorescence microscopy; gene therapy; histology; image processing; light microscopy; tissue /cell culture; transfection /expression vector

This Program Project represents a cohesive group of laboratories and investigators that share common goals and strategies focussing on the use of adenovirus-mediated gene therapy to the respiratory tract as a therapeutic tool for treating cystic fibrosis. These laboratories also share a need for several techniques including morphological analysis of data generated by gene transfer studies and specialty techniques for growing handling, and analyzing cell cultures to which genes are transferred. The Cell Biology Core provides to the investigators a variety of imaging and detection methods and specialty cell culture techniques to study interaction of vectors with target cells, expression of vector-encoded transgenes, and the physiological impact of transgene expression. Toward this end, the Cell Biology Core maintains state-of- the-art equipment for fluorescence and brightfield image acquisition, documentation, and presentation and facilitated aces to additional medical center resources such as histological, ultrastructural, and confocal facilities. The staff of the Cell Biology Core has extensive training in a variety of cytological, and cell culture techniques and will dedicate time and resources to make those assets available to the participating projects.

该计划项目代表了一个有凝聚力的实验室团体， 有着共同目标和战略的研究人员， 使用腺病毒介导的呼吸道基因治疗作为 用于治疗囊性纤维化的治疗工具。这些实验室还 共同需要几种技术，包括形态学分析， 基因转移研究和专业技术产生的数据， 生长处理和分析细胞培养物， 转移.细胞生物学核心为研究人员提供了一个 多种成像和检测方法以及特殊细胞培养 研究载体与靶细胞、表达 载体编码的转基因，以及转基因的生理影响 表情为此，细胞生物学核心保持了... 用于荧光和明场图像采集的现有技术设备， 文件和演示文稿，并促进aces以额外的 医学中心资源，如组织学，超微结构， 共焦设备。细胞生物学核心的工作人员有广泛的 培训各种细胞学和细胞培养技术， 将投入时间和资源，使这些资产提供给 参与项目。