CORE--CELL BIOLOGY

描述(由申请人提供)： 细胞生物学核心为计划项目研究人员提供集中的专业知识和设备，用于培养从不同物种、器官和分化阶段获得的内皮细胞，样品制备，以及用于图像采集和分析的最先进的形态学资源。总部设在遗传医学研究所的细胞生物学核心包括组织培养设施、配备显微镜和摄像系统的显微镜房间、湿实验室和暗室。除了维护设备和培训用户外，由沃尔特·范特·霍夫博士和菲利普·利奥波德博士共同指导的细胞生物学核心也将协助设计和规划实验策略。细胞生物学核心将分离和维护各种内皮细胞类型，如为项目6和8提供基本模型的人脐带内皮细胞和人皮肤和肺微血管内皮细胞，以及项目7中使用的小鼠新生心脏内皮细胞。此外，细胞生物学核心将通过免疫组织化学检测项目6、7、8和9中使用的转基因小鼠模型中的内皮细胞，帮助组织分析各种生长因子或病毒基因对分化、新生血管或缺血的影响。最后，细胞生物学核心将在项目6、8和9中提供用于评估腺病毒载体向内皮细胞转移基因的荧光Ad载体和其他载体的专家显微分析。

DESCRIPTION (provided by applicant): The Cell Biology Core provides Program Project investigators with centralized expertise and equipment for the culture of endothelial cells, acquired from different species, organs and differentiation stages, sample preparation, and state-of-the-art morphology resources for image acquisition and analysis. The Cell Biology Core, based in the Institute of Genetic Medicine, includes tissue culture facilities, microscopy rooms with microscopes and camera systems, wet laboratories, and a darkroom. In addition to maintaining equipment and training users, the Cell Biology Core, which is co-directed by Wouter van't Hof, Ph.D., and Philip Leopold, Ph.D., will also assist in the design and the planning of the experimental strategies. The Cell biology Core will isolate and maintain the various endothelial cell types, such as human umbilical cord endothelial cells and human dermal and lung microvascular endothelial cells that provide the basic models for Projects 6 and 8, as well as the mouse newborn cardiac endothelial cells used in Project 7. In addition, the Cell Biology Core will assist in the histological analysis of the effects of various growth factors or viral genes on differentiation, neovascularization or ischemia, by immunohistochemical detection of endothelial cells in transgenic mouse models used in Projects 6, 7, 8, and 9. Finally, the Cell Biology Core will provide expert microscopic analysis of fluorescent Ad vectors and other vectors used in the assessment of gene transfer to endothelial cells by adenovirus vectors in Projects 6, 8 and 9.